2g1p

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{{Seed}}
 
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[[Image:2g1p.png|left|200px]]
 
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==Structure of E. coli DNA adenine methyltransferase (DAM)==
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The line below this paragraph, containing "STRUCTURE_2g1p", creates the "Structure Box" on the page.
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<StructureSection load='2g1p' size='340' side='right'caption='[[2g1p]], [[Resolution|resolution]] 1.89&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2g1p]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G1P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2G1P FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.89&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
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{{STRUCTURE_2g1p| PDB=2g1p | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2g1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g1p OCA], [https://pdbe.org/2g1p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2g1p RCSB], [https://www.ebi.ac.uk/pdbsum/2g1p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2g1p ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DMA_ECOLI DMA_ECOLI] Methylates DNA within the sequence GATC and protects the DNA from cleavage by the restriction endonuclease MboI. Although it shares sequence specificity with a number of type II restriction endonucleases and methylases, it is thought to act in postreplication mismatch repair rather than as a part of a restriction modification system. May also play a role in DNA replication.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g1/2g1p_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2g1p ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The structure of the Escherichia coli Dam DNA-(adenine-N6)-methyltransferase in complex with cognate DNA was determined at 1.89 A resolution in the presence of S-adenosyl-L-homocysteine. DNA recognition and the dynamics of base-flipping were studied by site-directed mutagenesis, DNA methylation kinetics and fluorescence stopped-flow experiments. Our data illustrate the mechanism of coupling of DNA recognition and base-flipping. Contacts to the non-target strand in the second (3') half of the GATC site are established by R124 to the fourth base-pair, and by L122 and P134 to the third base-pair. The aromatic ring of Y119 intercalates into the DNA between the second and third base-pairs, which is essential for base-flipping to occur. Compared to previous published structures of bacteriophage T4 Dam, three major new observations are made in E.coli Dam. (1) The first Gua is recognized by K9, removal of which abrogates the first base-pair recognition. (2) The flipped target Ade binds to the surface of EcoDam in the absence of S-adenosyl-L-methionine, which illustrates a possible intermediate in the base-flipping pathway. (3) The orphaned Thy residue displays structural flexibility by adopting an extrahelical or intrahelical position where it is in contact to N120.
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===Structure of E. coli DNA adenine methyltransferase (DAM)===
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Structure and substrate recognition of the Escherichia coli DNA adenine methyltransferase.,Horton JR, Liebert K, Bekes M, Jeltsch A, Cheng X J Mol Biol. 2006 Apr 28;358(2):559-70. Epub 2006 Feb 28. PMID:16524590<ref>PMID:16524590</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2g1p" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_16524590}}, adds the Publication Abstract to the page
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*[[DNA adenine methylase|DNA adenine methylase]]
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(as it appears on PubMed at http://www.pubmed.gov), where 16524590 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16524590}}
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__TOC__
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</StructureSection>
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==About this Structure==
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2G1P is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G1P OCA].
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==Reference==
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<ref group="xtra">PMID:16524590</ref><references group="xtra"/>
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Bekes, M.]]
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[[Category: Large Structures]]
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[[Category: Cheng, X.]]
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[[Category: Bekes M]]
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[[Category: Horton, J R.]]
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[[Category: Cheng X]]
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[[Category: Jeltsch, A.]]
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[[Category: Horton JR]]
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[[Category: Liebert, K.]]
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[[Category: Jeltsch A]]
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[[Category: Bacterial virulence factor]]
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[[Category: Liebert K]]
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[[Category: Base flipping]]
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[[Category: Dam methylation]]
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[[Category: Gatc recognition]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 19:25:12 2009''
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Current revision

Structure of E. coli DNA adenine methyltransferase (DAM)

PDB ID 2g1p

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