1xt9

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{{Seed}}
 
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[[Image:1xt9.png|left|200px]]
 
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==Crystal Structure of Den1 in complex with Nedd8==
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The line below this paragraph, containing "STRUCTURE_1xt9", creates the "Structure Box" on the page.
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<StructureSection load='1xt9' size='340' side='right'caption='[[1xt9]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1xt9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XT9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XT9 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xt9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xt9 OCA], [https://pdbe.org/1xt9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xt9 RCSB], [https://www.ebi.ac.uk/pdbsum/1xt9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xt9 ProSAT]</span></td></tr>
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{{STRUCTURE_1xt9| PDB=1xt9 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NEDD8_HUMAN NEDD8_HUMAN] Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.<ref>PMID:10318914</ref> <ref>PMID:10597293</ref> <ref>PMID:11953428</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xt/1xt9_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xt9 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The Nedd8 conjugation pathway is conserved from yeast to humans and is essential in many organisms. Nedd8 is conjugated to cullin proteins in a process that alters SCF E3 ubiquitin ligase activity, and it is presumed that Nedd8 deconjugation would reverse these effects. We now report the X-ray structures of the human Nedd8-specific protease, Den1, in a complex with the inhibitor Nedd8 aldehyde, thus revealing a model for the tetrahedral transition state intermediate generated during proteolysis. Although Den1 is closely related to the SUMO-specific protease family (Ulp/Senp family), structural analysis of the interface suggests determinants involved in Nedd8 selectivity by Den1 over other ubiquitin-like family members and suggests how the Ulp/Senp architecture has been modified to interact with different ubiquitin-like modifiers.
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===Crystal Structure of Den1 in complex with Nedd8===
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Structure of a complex between Nedd8 and the Ulp/Senp protease family member Den1.,Reverter D, Wu K, Erdene TG, Pan ZQ, Wilkinson KD, Lima CD J Mol Biol. 2005 Jan 7;345(1):141-51. PMID:15567417<ref>PMID:15567417</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1xt9" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_15567417}}, adds the Publication Abstract to the page
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*[[NEDD8|NEDD8]]
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(as it appears on PubMed at http://www.pubmed.gov), where 15567417 is the PubMed ID number.
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*[[Sentrin-specific protease|Sentrin-specific protease]]
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== References ==
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{{ABSTRACT_PUBMED_15567417}}
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<references/>
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__TOC__
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==About this Structure==
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</StructureSection>
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1XT9 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XT9 OCA].
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==Reference==
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<ref group="xtra">PMID:15567417</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Erdene, T G.]]
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[[Category: Large Structures]]
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[[Category: Lima, C D.]]
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[[Category: Erdene TG]]
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[[Category: Pan, Z Q.]]
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[[Category: Lima CD]]
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[[Category: Reverter, D.]]
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[[Category: Pan ZQ]]
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[[Category: Wilkinson, K D.]]
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[[Category: Reverter D]]
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[[Category: Wu, K.]]
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[[Category: Wilkinson KD]]
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[[Category: Cysteine protease]]
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[[Category: Wu K]]
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[[Category: Ubiquitin-like]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 19:45:07 2009''
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Current revision

Crystal Structure of Den1 in complex with Nedd8

PDB ID 1xt9

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OCA

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