3hsf

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{{Seed}}
 
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[[Image:3hsf.png|left|200px]]
 
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==HEAT SHOCK TRANSCRIPTION FACTOR (HSF)==
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The line below this paragraph, containing "STRUCTURE_3hsf", creates the "Structure Box" on the page.
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<StructureSection load='3hsf' size='340' side='right'caption='[[3hsf]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3hsf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2hsf 2hsf]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HSF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HSF FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hsf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hsf OCA], [https://pdbe.org/3hsf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hsf RCSB], [https://www.ebi.ac.uk/pdbsum/3hsf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hsf ProSAT]</span></td></tr>
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{{STRUCTURE_3hsf| PDB=3hsf | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HSF_KLULA HSF_KLULA] DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. Also required for growth at normal temperatures.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hs/3hsf_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hsf ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The solution structure of the 92 residue (11 kDa) winged helix-turn-helix DNA-binding domain from the kluyveromyces lactis heat shock factor was refined using a total of 932 NOE, 35 phi, 25 chi 1, 5 chi 2 and 44 hydrogen bond restraints. The overall root-mean-square deviation for structured regions was 0.75(+/- 0.15) A. The three-helix bundle and four-stranded beta-sheet are well defined with rmsd of 0.53(+/- 0.10) A and 0.60(+/- 0.17) A, respectively. Helix H2 is underwound and bent near Pro45. The angle between helix H2 and the proposed recognition helix H3 is 96(+/- 6) degrees. Detailed comparisons are made with the X-ray structure of this protein as well as other structural studies on HSF. Overall, the results are consistent with the earlier studies. Differences are related to protein-protein interactions in the crystal and dynamics in solution. Backbone dynamics was investigated via 15N relaxation. The average R1, R2 and NOE values for residues in segments of secondary structure were 1.9(+/- 0.9) s-1, 7.8(+/- 0.9) s-1 and 0.81(+/- 0.05), respectively. The correlation time based on these data was 5.6(+/- 0.4) ns. Motional order parameters were calculated by fitting the relaxation data to one of three models. Low-order parameters were found for residues that comprise the turn between helices H2 and H3 (residues Lys49 to Phe53), and most strikingly, the 16 residue wing (residues Val68 to Arg83). These data are consistent with the lack of long-range NOEs identified in these regions. The data provide a basis for comparison with results of the protein-DNA complex. The relationship between structure and function is discussed.
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===HEAT SHOCK TRANSCRIPTION FACTOR (HSF)===
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Refined solution structure and dynamics of the DNA-binding domain of the heat shock factor from Kluyveromyces lactis.,Damberger FF, Pelton JG, Liu C, Cho H, Harrison CJ, Nelson HC, Wemmer DE J Mol Biol. 1995 Dec 8;254(4):704-19. PMID:7500344<ref>PMID:7500344</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3hsf" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_7500344}}, adds the Publication Abstract to the page
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*[[Heat shock factor|Heat shock factor]]
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(as it appears on PubMed at http://www.pubmed.gov), where 7500344 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_7500344}}
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__TOC__
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</StructureSection>
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==About this Structure==
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3HSF is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2hsf 2hsf]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HSF OCA].
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==Reference==
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<ref group="xtra">PMID:7500344</ref><references group="xtra"/>
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[[Category: Kluyveromyces lactis]]
[[Category: Kluyveromyces lactis]]
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[[Category: Cho, H.]]
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[[Category: Large Structures]]
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[[Category: Damberger, F F.]]
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[[Category: Cho H]]
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[[Category: Harrison, C J.]]
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[[Category: Damberger FF]]
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[[Category: Liu, C.]]
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[[Category: Harrison CJ]]
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[[Category: Nelson, H C.M.]]
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[[Category: Liu C]]
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[[Category: Pelton, J G.]]
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[[Category: Nelson HCM]]
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[[Category: Wemmer, D E.]]
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[[Category: Pelton JG]]
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[[Category: Transcription regulation]]
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[[Category: Wemmer DE]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 19:59:41 2009''
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Current revision

HEAT SHOCK TRANSCRIPTION FACTOR (HSF)

PDB ID 3hsf

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