1xua

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{{Seed}}
 
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[[Image:1xua.png|left|200px]]
 
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==Structure and function of the phenazine biosynthetic protein PhzF from Pseudomonas fluorescens==
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The line below this paragraph, containing "STRUCTURE_1xua", creates the "Structure Box" on the page.
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<StructureSection load='1xua' size='340' side='right'caption='[[1xua]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1xua]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XUA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XUA FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HHA:(2S,3S)-TRANS-2,3-DIHYDRO-3-HYDROXYANTHRANILIC+ACID'>HHA</scene></td></tr>
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{{STRUCTURE_1xua| PDB=1xua | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xua OCA], [https://pdbe.org/1xua PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xua RCSB], [https://www.ebi.ac.uk/pdbsum/1xua PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xua ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PHZF_PSEFL PHZF_PSEFL] Isomerase that catalyzes the condensation of two molecules of trans-2,3-dihydro-3-hydroxyanthranilic acid (DHHA) into the phenazine ring system. The final product is not yet known.<ref>PMID:19053436</ref> <ref>PMID:15545603</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xu/1xua_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xua ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Phenazines produced by Pseudomonas and Streptomyces spp. are heterocyclic nitrogen-containing metabolites with antibiotic, antitumor, and antiparasitic activity. The antibiotic properties of pyocyanin, produced by Pseudomonas aeruginosa, were recognized in the 1890s, although this blue phenazine is now known to be a virulence factor in human disease. Despite their biological significance, the biosynthesis of phenazines is not fully understood. Here we present structural and functional studies of PhzF, an enzyme essential for phenazine synthesis in Pseudomonas spp. PhzF shares topology with diaminopimelate epimerase DapF but lacks the same catalytic residues. The structure of PhzF in complex with its substrate, trans-2,3-dihydro-3-hydroxyanthranilic acid, suggests that it is an isomerase using the conserved glutamate E45 to abstract a proton from C3 of the substrate. The proton is returned to C1 of the substrate after rearrangement of the double-bond system, yielding an enol that converts to the corresponding ketone. PhzF is a dimer that may be bifunctional, providing a shielded cavity for ketone dimerization via double Schiff-base formation to produce the phenazine scaffold. Our proposed mechanism is supported by mass and NMR spectroscopy. The results are discussed in the context of related structures and protein sequences of unknown biochemical function.
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===Structure and function of the phenazine biosynthetic protein PhzF from Pseudomonas fluorescens===
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Structure and function of the phenazine biosynthetic protein PhzF from Pseudomonas fluorescens.,Blankenfeldt W, Kuzin AP, Skarina T, Korniyenko Y, Tong L, Bayer P, Janning P, Thomashow LS, Mavrodi DV Proc Natl Acad Sci U S A. 2004 Nov 23;101(47):16431-6. Epub 2004 Nov 15. PMID:15545603<ref>PMID:15545603</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_15545603}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1xua" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 15545603 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15545603}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1XUA is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XUA OCA].
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==Reference==
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<ref group="xtra">PMID:15545603</ref><references group="xtra"/>
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[[Category: Pseudomonas fluorescens]]
[[Category: Pseudomonas fluorescens]]
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[[Category: Bayer, P.]]
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[[Category: Bayer P]]
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[[Category: Blankenfeldt, W.]]
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[[Category: Blankenfeldt W]]
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[[Category: Janning, P.]]
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[[Category: Janning P]]
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[[Category: Korniyenko, Y.]]
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[[Category: Korniyenko Y]]
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[[Category: Kuzin, A P.]]
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[[Category: Kuzin AP]]
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[[Category: Mavrodi, D V.]]
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[[Category: Mavrodi DV]]
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[[Category: Skarina, T.]]
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[[Category: Skarina T]]
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[[Category: Thomashow, L S.]]
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[[Category: Thomashow LS]]
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[[Category: Tong, L.]]
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[[Category: Tong L]]
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[[Category: Phenazine biosynthesis]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 20:01:11 2009''
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Current revision

Structure and function of the phenazine biosynthetic protein PhzF from Pseudomonas fluorescens

PDB ID 1xua

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