1btb

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{{Seed}}
 
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[[Image:1btb.png|left|200px]]
 
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==THREE-DIMENSIONAL SOLUTION STRUCTURE AND 13C ASSIGNMENTS OF BARSTAR USING NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY==
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The line below this paragraph, containing "STRUCTURE_1btb", creates the "Structure Box" on the page.
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<StructureSection load='1btb' size='340' side='right'caption='[[1btb]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1btb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BTB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BTB FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1btb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1btb OCA], [https://pdbe.org/1btb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1btb RCSB], [https://www.ebi.ac.uk/pdbsum/1btb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1btb ProSAT]</span></td></tr>
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{{STRUCTURE_1btb| PDB=1btb | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/BARS_BACAM BARS_BACAM] Inhibitor of the ribonuclease barnase. Forms a one-to-one non-covalent complex.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bt/1btb_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1btb ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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We present the high-resolution solution structure and 13C assignments of wild-type barstar, an 89 amino acid residue polypeptide inhibitor of barnase, derived from heteronuclear NMR techniques. These were obtained from measurements on unlabeled, uniformly 15N- and 13C/15N-labeled, and 10% 13C-labeled barstar samples that have both cysteines (at positions 40 and 82) fully reduced. In total, 30 structures were calculated by hybrid distance geometry-dynamical simulated annealing calculations. The atomic rms distribution about the mean coordinate positions is 0.42 A for all backbone atoms and 0.90 A for all atoms. The structure is composed of three parallel alpha-helices packed against a three-stranded parallel beta-sheet. A more poorly defined helix links the second beta-strand and the third major alpha-helix. The loop involved in binding barnase is extremely well defined and held rigidly by interactions from the main body of the protein to both ends and the middle of the loop. This structure will be used to aid protein engineering studies currently taking place on the free and bound states of barstar and barnase.
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===THREE-DIMENSIONAL SOLUTION STRUCTURE AND 13C ASSIGNMENTS OF BARSTAR USING NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY===
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Three-dimensional solution structure and 13C assignments of barstar using nuclear magnetic resonance spectroscopy.,Lubienski MJ, Bycroft M, Freund SM, Fersht AR Biochemistry. 1994 Aug 2;33(30):8866-77. PMID:8043574<ref>PMID:8043574</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1btb" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_8043574}}, adds the Publication Abstract to the page
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*[[Barstar 3D structures|Barstar 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 8043574 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_8043574}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1BTB is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BTB OCA].
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==Reference==
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<ref group="xtra">PMID:8043574</ref><references group="xtra"/>
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[[Category: Bacillus amyloliquefaciens]]
[[Category: Bacillus amyloliquefaciens]]
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[[Category: Bycroft, M.]]
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[[Category: Large Structures]]
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[[Category: Fersht, A R.]]
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[[Category: Bycroft M]]
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[[Category: Freund, S M.V.]]
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[[Category: Fersht AR]]
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[[Category: Lubienski, M J.]]
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[[Category: Freund SMV]]
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[[Category: Ribonuclease inhibitor]]
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[[Category: Lubienski MJ]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 20:05:27 2009''
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Current revision

THREE-DIMENSIONAL SOLUTION STRUCTURE AND 13C ASSIGNMENTS OF BARSTAR USING NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY

PDB ID 1btb

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