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1z9b

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(New page: 200px<br /><applet load="1z9b" size="450" color="white" frame="true" align="right" spinBox="true" caption="1z9b" /> '''Solution structure of the C1-subdomain of Ba...)
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[[Image:1z9b.gif|left|200px]]<br /><applet load="1z9b" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1z9b" />
 
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'''Solution structure of the C1-subdomain of Bacillus stearothermophilus translation initiation factor IF2'''<br />
 
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==Overview==
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==Solution structure of the C1-subdomain of Bacillus stearothermophilus translation initiation factor IF2==
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IF2 is one of three bacterial translation initiation factors that are, conserved through all kingdoms of life. It binds the 30S and 50S ribosomal, subunits, as well as fMet-tRNAf(Met). After these interactions, fMet-tRNAf(Met) is oriented to the ribosomal P-site where the first amino, acid of the nascent polypeptide, formylmethionine, is presented. The, C-terminal domain of Bacillus stearothermophilus IF2, which is responsible, for recognition and binding of fMet-tRNAf(Met), contains two structured, modules. Previously, the solution structure of the most C-terminal module, IF2-C2, has been elucidated by NMR spectroscopy and direct interactions, between this subdomain and fMet-tRNAf(Met) were reported. In the present, NMR study we have obtained the spectral assignment of the other module of, the C-terminal domain (IF2-C1) and determined its solution structure and, backbone dynamics. The IF2-C1 core forms a flattened fold consisting of a, central four-stranded parallel beta-sheet flanked by three alpha-helices., Although its overall organization resembles that of subdomain III of the, archaeal IF2-homolog eIF5B whose crystal structure had previously been, reported, some differences of potential functional significance are, evident.
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<StructureSection load='1z9b' size='340' side='right'caption='[[1z9b]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1z9b]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z9B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Z9B FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1z9b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z9b OCA], [https://pdbe.org/1z9b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1z9b RCSB], [https://www.ebi.ac.uk/pdbsum/1z9b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1z9b ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IF2_GEOSE IF2_GEOSE] One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z9/1z9b_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1z9b ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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IF2 is one of three bacterial translation initiation factors that are conserved through all kingdoms of life. It binds the 30S and 50S ribosomal subunits, as well as fMet-tRNAf(Met). After these interactions, fMet-tRNAf(Met) is oriented to the ribosomal P-site where the first amino acid of the nascent polypeptide, formylmethionine, is presented. The C-terminal domain of Bacillus stearothermophilus IF2, which is responsible for recognition and binding of fMet-tRNAf(Met), contains two structured modules. Previously, the solution structure of the most C-terminal module, IF2-C2, has been elucidated by NMR spectroscopy and direct interactions between this subdomain and fMet-tRNAf(Met) were reported. In the present NMR study we have obtained the spectral assignment of the other module of the C-terminal domain (IF2-C1) and determined its solution structure and backbone dynamics. The IF2-C1 core forms a flattened fold consisting of a central four-stranded parallel beta-sheet flanked by three alpha-helices. Although its overall organization resembles that of subdomain III of the archaeal IF2-homolog eIF5B whose crystal structure had previously been reported, some differences of potential functional significance are evident.
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==About this Structure==
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Solution structure of the C1-subdomain of Bacillus stearothermophilus translation initiation factor IF2.,Wienk H, Tomaselli S, Bernard C, Spurio R, Picone D, Gualerzi CO, Boelens R Protein Sci. 2005 Sep;14(9):2461-8. Epub 2005 Aug 4. PMID:16081655<ref>PMID:16081655</ref>
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1Z9B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1Z9B OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Solution structure of the C1-subdomain of Bacillus stearothermophilus translation initiation factor IF2., Wienk H, Tomaselli S, Bernard C, Spurio R, Picone D, Gualerzi CO, Boelens R, Protein Sci. 2005 Sep;14(9):2461-8. Epub 2005 Aug 4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16081655 16081655]
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</div>
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<div class="pdbe-citations 1z9b" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Geobacillus stearothermophilus]]
[[Category: Geobacillus stearothermophilus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bernard, C.]]
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[[Category: Bernard C]]
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[[Category: Boelens, R.]]
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[[Category: Boelens R]]
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[[Category: Gualerzi, C.O.]]
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[[Category: Gualerzi CO]]
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[[Category: Picone, D.]]
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[[Category: Picone D]]
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[[Category: Spurio, R.]]
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[[Category: Spurio R]]
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[[Category: Tomaselli, S.]]
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[[Category: Tomaselli S]]
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[[Category: Wienk, H.]]
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[[Category: Wienk H]]
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[[Category: protein synthesis translation intiation if2 fmet-trna nmr structure]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:19:00 2007''
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Current revision

Solution structure of the C1-subdomain of Bacillus stearothermophilus translation initiation factor IF2

PDB ID 1z9b

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