1np4

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{{Seed}}
 
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[[Image:1np4.png|left|200px]]
 
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==CRYSTAL STRUCTURE OF NITROPHORIN 4 FROM RHODNIUS PROLIXUS==
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The line below this paragraph, containing "STRUCTURE_1np4", creates the "Structure Box" on the page.
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<StructureSection load='1np4' size='340' side='right'caption='[[1np4]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1np4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodnius_prolixus Rhodnius prolixus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NP4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NP4 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=NH3:AMMONIA'>NH3</scene></td></tr>
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{{STRUCTURE_1np4| PDB=1np4 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1np4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1np4 OCA], [https://pdbe.org/1np4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1np4 RCSB], [https://www.ebi.ac.uk/pdbsum/1np4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1np4 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NP4_RHOPR NP4_RHOPR] Heme-based protein that deliver nitric oxide gas (NO) to the victim while feeding, resulting in vasodilation and inhibition of platelet aggregation. Also bind tightly to histamine, which is released by the host to induce wound healing (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/np/1np4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1np4 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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BACKGROUND: Nitrophorins are nitric oxide (NO) transport proteins from the saliva of blood-feeding insects, which act as vasodilators and anti-platelet agents. Rhodnius prolixus, an insect that carries the trypanosome that causes Chagas' disease, releases four NO-loaded nitrophorins during blood feeding, whereupon the ligand is released into the bloodstream or surrounding tissue of the host. Histamine, a signaling molecule released by the host upon tissue damage, is tightly bound by the nitrophorins; this may facilitate the release of NO and reduce inflammation in the host. RESULTS: Recombinant nitrophorin 4 (NP4) was expressed in Escherichia coli, reconstituted with heme, and found to bind NO and histamine in a manner similar to that of the natural protein. The crystal structure of NP4 revealed a lipocalin-like eight-stranded beta barrel, with heme inserted into one end of the barrel. His59 ligates the proximal site on the heme, a solvent molecule (NH3) ligates the distal site, and three additional solvent molecules occupy the distal pocket. Buried in the protein interior are Glu55 and three solvent molecules. A detailed comparison with other lipocalins suggests that NP4 is closely related to the biliverdin-binding proteins from insects. CONCLUSIONS: The nitrophorins have a unique hemoprotein structure and are completely unlike the globins, the only other hemoproteins designed to transport dissolved gases. Compared with the recently described structure of NP1, the NP4 structure is considerably higher resolution, confirms the unusual placement of ionizable groups in the protein interior, and clarifies the solvent arrangement in the distal pocket. It also provides a striking example of structural homology where sequence homology is minimal.
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===CRYSTAL STRUCTURE OF NITROPHORIN 4 FROM RHODNIUS PROLIXUS===
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The crystal structure of nitrophorin 4 at 1.5 A resolution: transport of nitric oxide by a lipocalin-based heme protein.,Andersen JF, Weichsel A, Balfour CA, Champagne DE, Montfort WR Structure. 1998 Oct 15;6(10):1315-27. PMID:9782054<ref>PMID:9782054</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1np4" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_9782054}}, adds the Publication Abstract to the page
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*[[Nitrophorin|Nitrophorin]]
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(as it appears on PubMed at http://www.pubmed.gov), where 9782054 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_9782054}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1NP4 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Rhodnius_prolixus Rhodnius prolixus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NP4 OCA].
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==Reference==
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<ref group="xtra">PMID:9782054</ref><references group="xtra"/>
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[[Category: Rhodnius prolixus]]
[[Category: Rhodnius prolixus]]
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[[Category: Andersen, J F.]]
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[[Category: Andersen JF]]
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[[Category: Balfour, C A.]]
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[[Category: Balfour CA]]
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[[Category: Champagne, D E.]]
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[[Category: Champagne DE]]
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[[Category: Montfort, W R.]]
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[[Category: Montfort WR]]
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[[Category: Weichsel, A.]]
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[[Category: Weichsel A]]
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[[Category: Antihistamine]]
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[[Category: Bilin binding protein]]
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[[Category: Ferric heme]]
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[[Category: Lipocalin]]
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[[Category: Nitric oxide transport]]
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[[Category: Vasodilator]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 20:21:05 2009''
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Current revision

CRYSTAL STRUCTURE OF NITROPHORIN 4 FROM RHODNIUS PROLIXUS

PDB ID 1np4

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