2ckk

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{{Seed}}
 
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[[Image:2ckk.png|left|200px]]
 
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==High resolution crystal structure of the human kin17 C-terminal domain containing a kow motif==
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The line below this paragraph, containing "STRUCTURE_2ckk", creates the "Structure Box" on the page.
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<StructureSection load='2ckk' size='340' side='right'caption='[[2ckk]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2ckk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CKK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CKK FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.45&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>
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{{STRUCTURE_2ckk| PDB=2ckk | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ckk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ckk OCA], [https://pdbe.org/2ckk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ckk RCSB], [https://www.ebi.ac.uk/pdbsum/2ckk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ckk ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/KIN17_HUMAN KIN17_HUMAN] Involved in DNA replication and the cellular response to DNA damage. May participate in DNA replication factories and create a bridge between DNA replication and repair mediated by high molecular weight complexes. May play a role in illegitimate recombination and regulation of gene expression. May participate in mRNA processing. Binds, in vitro, to double-stranded DNA. Also shown to bind preferentially to curved DNA in vitro and in vivo (By similarity). Binds via its C-terminal domain to RNA in vitro.[UniProtKB:Q8K339]<ref>PMID:11880372</ref> <ref>PMID:12359749</ref> <ref>PMID:12754299</ref> <ref>PMID:12853634</ref> <ref>PMID:15831485</ref> <ref>PMID:17045609</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ck/2ckk_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ckk ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The human KIN17 protein is an essential nuclear protein conserved from yeast to human and expressed ubiquitously in mammals. Suppression of Rts2, the yeast equivalent of gene KIN17, renders the cells unviable, and silencing the human KIN17 gene slows cell growth dramatically. Moreover, the human gene KIN17 is up-regulated following exposure to ionizing radiations and UV light, depending on the integrity of the human global genome repair machinery. Its ectopic over-expression blocks S-phase progression by inhibiting DNA synthesis. The C-terminal region of human KIN17 is crucial for this anti-proliferation effect. Its high-resolution structure, presented here, reveals a tandem of SH3-like subdomains. This domain binds to ribonucleotide homopolymers with the same preferences as the whole protein. Analysis of its structure complexed with tungstate shows structural variability within the domain. The interaction with tungstate is mediated by several lysine residues located within a positively charged groove at the interface between the two subdomains. This groove could be the site of interaction with RNA, since mutagenesis of two of these highly conserved lysine residue weakens RNA binding.
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===HIGH RESOLUTION CRYSTAL STRUCTURE OF THE HUMAN KIN17 C-TERMINAL DOMAIN CONTAINING A KOW MOTIF KIN17.===
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A tandem of SH3-like domains participates in RNA binding in KIN17, a human protein activated in response to genotoxics.,le Maire A, Schiltz M, Stura EA, Pinon-Lataillade G, Couprie J, Moutiez M, Gondry M, Angulo JF, Zinn-Justin S J Mol Biol. 2006 Dec 8;364(4):764-76. Epub 2006 Sep 16. PMID:17045609<ref>PMID:17045609</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==About this Structure==
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</div>
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2CKK is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CKK OCA].
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<div class="pdbe-citations 2ckk" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Angulo-Mora, J.]]
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[[Category: Large Structures]]
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[[Category: Couprie, J.]]
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[[Category: Angulo-Mora J]]
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[[Category: Gondry, M.]]
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[[Category: Couprie J]]
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[[Category: Maire, A Le.]]
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[[Category: Gondry M]]
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[[Category: Pinon-Lataillade, G.]]
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[[Category: Pinon-Lataillade G]]
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[[Category: Schiltz, M.]]
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[[Category: Schiltz M]]
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[[Category: Stura, E.]]
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[[Category: Stura E]]
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[[Category: Zinn-Justin, S.]]
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[[Category: Zinn-Justin S]]
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[[Category: Beta barrel]]
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[[Category: Le Maire A]]
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[[Category: Nuclear protein]]
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[[Category: Ribonucleoprotein]]
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[[Category: Ribosomal protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 20:41:30 2009''
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Current revision

High resolution crystal structure of the human kin17 C-terminal domain containing a kow motif

PDB ID 2ckk

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