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1zcd

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Crystal structure of the Na+/H+ antiporter NhaA

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Retrieved from "http://52.214.119.220/wiki/index.php/1zcd"

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-[[Image:1zcd.gif|left|200px]]<br /><applet load="1zcd" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1zcd, resolution 3.45&Aring;" />
-'''Crystal structure of the Na+/H+ antiporter NhaA'''<br />
-==Overview==
+==Crystal structure of the Na+/H+ antiporter NhaA==
-The control by Na+/H+ antiporters of sodium/proton concentration and cell, volume is crucial for the viability of all cells. Adaptation to high, salinity and/or extreme pH in plants and bacteria or in human heart, muscles requires the action of Na+/H+ antiporters. Their activity is, tightly controlled by pH. Here we present the crystal structure of, pH-downregulated NhaA, the main antiporter of Escherichia coli and many, enterobacteria. A negatively charged ion funnel opens to the cytoplasm and, ends in the middle of the membrane at the putative ion-binding site., There, a unique assembly of two pairs of short helices connected by, crossed, extended chains creates a balanced electrostatic environment. We, propose that the binding of charged substrates causes an electric, imbalance, inducing movements, that permit a rapid alternating-access, mechanism. This ion-exchange machinery is regulated by a conformational, change elicited by a pH signal perceived at the entry to the cytoplasmic, funnel.
+<StructureSection load='1zcd' size='340' side='right'caption='[[1zcd]], [[Resolution|resolution]] 3.45&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[1zcd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. The December 2011 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Complex I''  by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2011_12 10.2210/rcsb_pdb/mom_2011_12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZCD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZCD FirstGlance]. <br>
-ZCD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ZCD OCA].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.45&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zcd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zcd OCA], [https://pdbe.org/1zcd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zcd RCSB], [https://www.ebi.ac.uk/pdbsum/1zcd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zcd ProSAT]</span></td></tr>
-==Reference==
+</table>
-Structure of a Na+/H+ antiporter and insights into mechanism of action and regulation by pH., Hunte C, Screpanti E, Venturi M, Rimon A, Padan E, Michel H, Nature. 2005 Jun 30;435(7046):1197-202. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15988517 15988517]
+== Function ==
+[https://www.uniprot.org/uniprot/NHAA_ECOLI NHAA_ECOLI] Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons. Catalyzes the exchange of 2 H(+) per Na(+). Can mediate sodium uptake when a transmembrane pH gradient is applied. Active at alkaline pH. Activity is strongly down-regulated below pH 6.5.<ref>PMID:1645730</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zc/1zcd_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zcd ConSurf].
+<div style="clear:both"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Complex I]]
 [[Category: Escherichia coli]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Hunte, C.]]
+[[Category: RCSB PDB Molecule of the Month]]
-[[Category: Michel, H.]]
+[[Category: Hunte C]]
-[[Category: Padan, E.]]
+[[Category: Michel H]]
-[[Category: Rimon, A.]]
+[[Category: Padan E]]
-[[Category: Screpanti, E.]]
+[[Category: Rimon A]]
-[[Category: Venturi, M.]]
+[[Category: Screpanti E]]
-[[Category: antiporter]]
+[[Category: Venturi M]]
-[[Category: membrane protein]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:22:56 2007''

1zcd

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Crystal structure of the Na+/H+ antiporter NhaA

Structural highlights

Function

Evolutionary Conservation

References

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