1hss

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{{Seed}}
 
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[[Image:1hss.png|left|200px]]
 
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==0.19 ALPHA-AMYLASE INHIBITOR FROM WHEAT==
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The line below this paragraph, containing "STRUCTURE_1hss", creates the "Structure Box" on the page.
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<StructureSection load='1hss' size='340' side='right'caption='[[1hss]], [[Resolution|resolution]] 2.06&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1hss]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HSS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HSS FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.06&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hss OCA], [https://pdbe.org/1hss PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hss RCSB], [https://www.ebi.ac.uk/pdbsum/1hss PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hss ProSAT]</span></td></tr>
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{{STRUCTURE_1hss| PDB=1hss | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IAA1_WHEAT IAA1_WHEAT] Alpha-amylase inhibitor.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hs/1hss_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hss ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of 0.19 alpha-amylase inhibitor (0.19 AI) from wheat kernel was determined by the multiple-isomorphous replacement method coupled with density modification and noncrystallographic symmetry averaging and then refined by simulated annealing using diffraction data to 2.06 A resolution (R = 18.7%, free R = 22.3%). The asymmetric unit has four molecules of 0.19 AI, each comprised of 124 amino acid residues. Electron density for residues 1-4 and 69-77 is absent in all subunits, probably because of the intrinsic flexibility of these segments. Each subunit has four major alpha-helices and one one-turn helix which are arranged in the up-and-down manner, maintaining the favorable packing modes of the alpha-helices. 0.19 AI, however, has two short antiparallel beta-strands. All 10 cysteine residues in 0.19 AI form disulfide bonds (C6-C52, C20-C41, C28-C83, C42-C99, and C54-C115), consistent with the assignments made biochemically for 0.28 AI from wheat kernel and by NMR analysis of the bifunctional alpha-amylase/trypsin inhibitor from ragi seeds (RBI). The disulfide bond patterns in these AIs are similar to those in the hydrophobic protein from soybean (HPS), which lack only the bond corresponding to C28-C83 in 0.19 AI. Extensive interactions occurred between particular pairs of 0.19 AI subunits, mainly involving hydrophobic residues. Comparisons of the structures of 0.19 AI, RBI, and HPS showed that the arrangements of the major alpha-helices are similar but the conformations of the remaining residues differ markedly. The present X-ray analysis for 0.19 AI and the NMR analysis for RBI suggest that all the AIs in this family have a common fold. The alpha-amylase binding site is discussed on the basis of the tertiary and quaternary structures of 0.19 AI together with biochemical and spectroscopic data for AIs.
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===0.19 ALPHA-AMYLASE INHIBITOR FROM WHEAT===
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Tertiary and quaternary structures of 0.19 alpha-amylase inhibitor from wheat kernel determined by X-ray analysis at 2.06 A resolution.,Oda Y, Matsunaga T, Fukuyama K, Miyazaki T, Morimoto T Biochemistry. 1997 Nov 4;36(44):13503-11. PMID:9354618<ref>PMID:9354618</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_9354618}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1hss" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 9354618 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_9354618}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1HSS is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HSS OCA].
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==Reference==
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<ref group="xtra">PMID:9354618</ref><references group="xtra"/>
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[[Category: Triticum aestivum]]
[[Category: Triticum aestivum]]
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[[Category: Fukuyama, K.]]
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[[Category: Fukuyama K]]
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[[Category: Oda, Y.]]
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[[Category: Oda Y]]
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[[Category: Animal amylase]]
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[[Category: Cereal inhibitor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 20:50:40 2009''
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Current revision

0.19 ALPHA-AMYLASE INHIBITOR FROM WHEAT

PDB ID 1hss

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