1zeq

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(New page: 200px<br /><applet load="1zeq" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zeq, resolution 1.50&Aring;" /> '''1.5 A Structure of a...)
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[[Image:1zeq.gif|left|200px]]<br /><applet load="1zeq" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1zeq, resolution 1.50&Aring;" />
 
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'''1.5 A Structure of apo-CusF residues 6-88 from Escherichia coli'''<br />
 
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==Overview==
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==1.5 A Structure of apo-CusF residues 6-88 from Escherichia coli==
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We have determined the crystal structure of apo-CusF, a periplasmic, protein involved in copper and silver resistance in Escherichia coli. The, protein forms a five-stranded beta-barrel, classified as an OB-fold, which, is a unique topology for a copper-binding protein. NMR chemical shift, mapping experiments suggest that Cu(I) is bound by conserved residues H36, M47, and M49 located in beta-strands 2 and 3. These residues are clustered, at one end of the beta-barrel, and their side chains are oriented toward, the interior of the barrel. Cu(I) can be modeled into the apo-CusF, structure with only minimal structural changes using H36, M47, and M49 as, ligands. The unique structure and metal binding site of CusF are distinct, from those of previously characterized copper-binding proteins.
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<StructureSection load='1zeq' size='340' side='right'caption='[[1zeq]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1zeq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZEQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZEQ FirstGlance]. <br>
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1ZEQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ZEQ OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zeq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zeq OCA], [https://pdbe.org/1zeq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zeq RCSB], [https://www.ebi.ac.uk/pdbsum/1zeq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zeq ProSAT]</span></td></tr>
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==Reference==
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</table>
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A novel copper-binding fold for the periplasmic copper resistance protein CusF., Loftin IR, Franke S, Roberts SA, Weichsel A, Heroux A, Montfort WR, Rensing C, McEvoy MM, Biochemistry. 2005 Aug 9;44(31):10533-40. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16060662 16060662]
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== Function ==
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[https://www.uniprot.org/uniprot/CUSF_ECOLI CUSF_ECOLI] Part of a cation efflux system that mediates resistance to copper and silver. Binds one copper per polypeptide.<ref>PMID:11399769</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ze/1zeq_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zeq ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Franke, S.]]
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[[Category: Franke S]]
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[[Category: Heroux, A.]]
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[[Category: Heroux A]]
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[[Category: Loftin, I.R.]]
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[[Category: Loftin IR]]
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[[Category: McEvoy, M.M.]]
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[[Category: McEvoy MM]]
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[[Category: Montfort, W.R.]]
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[[Category: Montfort WR]]
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[[Category: Rensing, C.]]
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[[Category: Rensing C]]
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[[Category: Roberts, S.A.]]
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[[Category: Roberts SA]]
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[[Category: Weichsel, A.]]
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[[Category: Weichsel A]]
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[[Category: beta barrel]]
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[[Category: copper-binding]]
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[[Category: metallochaperone]]
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[[Category: ob-fold]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:25:04 2007''
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Current revision

1.5 A Structure of apo-CusF residues 6-88 from Escherichia coli

PDB ID 1zeq

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