1u0u

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{{Seed}}
 
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[[Image:1u0u.png|left|200px]]
 
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==An Aldol Switch Discovered in Stilbene Synthases Mediates Cyclization Specificity of Type III Polyketide Synthases: Pine stilbene synthase structure==
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The line below this paragraph, containing "STRUCTURE_1u0u", creates the "Structure Box" on the page.
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<StructureSection load='1u0u' size='340' side='right'caption='[[1u0u]], [[Resolution|resolution]] 2.11&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1u0u]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Pinus_sylvestris Pinus sylvestris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U0U FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.11&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u0u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u0u OCA], [https://pdbe.org/1u0u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u0u RCSB], [https://www.ebi.ac.uk/pdbsum/1u0u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u0u ProSAT]</span></td></tr>
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{{STRUCTURE_1u0u| PDB=1u0u | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DPSS_PINSY DPSS_PINSY]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u0/1u0u_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u0u ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Stilbene synthase (STS) and chalcone synthase (CHS) each catalyze the formation of a tetraketide intermediate from a CoA-tethered phenylpropanoid starter and three molecules of malonyl-CoA, but use different cyclization mechanisms to produce distinct chemical scaffolds for a variety of plant natural products. Here we present the first STS crystal structure and identify, by mutagenic conversion of alfalfa CHS into a functional stilbene synthase, the structural basis for the evolution of STS cyclization specificity in type III polyketide synthase (PKS) enzymes. Additional mutagenesis and enzymatic characterization confirms that electronic effects rather than steric factors balance competing cyclization specificities in CHS and STS. Finally, we discuss the problematic in vitro reconstitution of plant stilbenecarboxylate pathways, using insights from existing biomimetic polyketide cyclization studies to generate a novel mechanistic hypothesis to explain stilbenecarboxylate biosynthesis.
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===An Aldol Switch Discovered in Stilbene Synthases Mediates Cyclization Specificity of Type III Polyketide Synthases: Pine stilbene synthase structure===
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An aldol switch discovered in stilbene synthases mediates cyclization specificity of type III polyketide synthases.,Austin MB, Bowman ME, Ferrer JL, Schroder J, Noel JP Chem Biol. 2004 Sep;11(9):1179-94. PMID:15380179<ref>PMID:15380179</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_15380179}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1u0u" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 15380179 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15380179}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1U0U is a 6 chains structure of sequences from [http://en.wikipedia.org/wiki/Pinus_sylvestris Pinus sylvestris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0U OCA].
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==Reference==
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<ref group="xtra">PMID:15380179</ref><references group="xtra"/>
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[[Category: Pinus sylvestris]]
[[Category: Pinus sylvestris]]
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[[Category: Austin, M B.]]
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[[Category: Austin MB]]
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[[Category: Bowman, M E.]]
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[[Category: Bowman ME]]
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[[Category: Ferrer, J L.]]
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[[Category: Ferrer J-L]]
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[[Category: Noel, J P.]]
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[[Category: Noel JP]]
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[[Category: Schroder, J.]]
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[[Category: Schroder J]]
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[[Category: Aldol switch]]
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[[Category: Alpha-beta-alpha-beta-alpha fold]]
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[[Category: Catalytic triad]]
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[[Category: Condensing enzyme]]
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[[Category: Pk]]
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[[Category: Thiolase fold]]
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[[Category: Type iii polyketide synthase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 21:10:30 2009''
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Current revision

An Aldol Switch Discovered in Stilbene Synthases Mediates Cyclization Specificity of Type III Polyketide Synthases: Pine stilbene synthase structure

PDB ID 1u0u

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