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1zh0

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(New page: 200px<br /><applet load="1zh0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zh0, resolution 1.900&Aring;" /> '''Crystal Structure o...)
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[[Image:1zh0.gif|left|200px]]<br /><applet load="1zh0" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1zh0, resolution 1.900&Aring;" />
 
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'''Crystal Structure of L-3-(2-napthyl)alanine-tRNA synthetasein complex with L-3-(2-napthyl)alanine'''<br />
 
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==Overview==
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==Crystal Structure of L-3-(2-napthyl)alanine-tRNA synthetase in complex with L-3-(2-napthyl)alanine==
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Recently, tRNA aminoacyl-tRNA synthetase pairs have been evolved that, allow one to genetically encode a large array of unnatural amino acids in, both prokaryotic and eukaryotic organisms. We have determined the crystal, structures of two substrate-bound Methanococcus jannaschii tyrosyl, aminoacyl-tRNA synthetases that charge the unnatural amino acids, p-bromophenylalanine and 3-(2-naphthyl)alanine (NpAla). A comparison of, these structures with the substrate-bound WT synthetase, as well as a, mutant synthetase that charges p-acetylphenylalanine, shows that altered, specificity is due to both side-chain and backbone rearrangements within, the active site that modify hydrogen bonds and packing interactions with, substrate, as well as disrupt the alpha8-helix, which spans the WT active, site. The high degree of structural plasticity that is observed in these, aminoacyl-tRNA synthetases is rarely found in other mutant enzymes with, altered specificities and provides an explanation for the surprising, adaptability of the genetic code to novel amino acids.
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<StructureSection load='1zh0' size='340' side='right'caption='[[1zh0]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1zh0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZH0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZH0 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAL:BETA-(2-NAPHTHYL)-ALANINE'>NAL</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zh0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zh0 OCA], [https://pdbe.org/1zh0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zh0 RCSB], [https://www.ebi.ac.uk/pdbsum/1zh0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zh0 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SYY_METJA SYY_METJA] Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).<ref>PMID:10585437</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zh/1zh0_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zh0 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1ZH0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with NAL and TRS as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Tyrosine--tRNA_ligase Tyrosine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.1 6.1.1.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ZH0 OCA].
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*[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]]
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== References ==
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==Reference==
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<references/>
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Structural plasticity of an aminoacyl-tRNA synthetase active site., Turner JM, Graziano J, Spraggon G, Schultz PG, Proc Natl Acad Sci U S A. 2006 Apr 25;103(17):6483-8. Epub 2006 Apr 17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16618920 16618920]
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Methanocaldococcus jannaschii]]
[[Category: Methanocaldococcus jannaschii]]
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[[Category: Single protein]]
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[[Category: Graziano J]]
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[[Category: Tyrosine--tRNA ligase]]
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[[Category: Schultz PG]]
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[[Category: Graziano, J.]]
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[[Category: Spraggon G]]
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[[Category: Schultz, P.G.]]
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[[Category: Turner JM]]
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[[Category: Spraggon, G.]]
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[[Category: Turner, J.M.]]
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[[Category: NAL]]
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[[Category: TRS]]
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[[Category: npala]]
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[[Category: structural plasticity]]
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[[Category: trna synthetase]]
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[[Category: unnatural amino acid]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:26:53 2007''
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Current revision

Crystal Structure of L-3-(2-napthyl)alanine-tRNA synthetase in complex with L-3-(2-napthyl)alanine

PDB ID 1zh0

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