1zhh

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(New page: 200px<br /><applet load="1zhh" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zhh, resolution 1.94&Aring;" /> '''Crystal Structure of...)
Current revision (09:05, 14 February 2024) (edit) (undo)
 
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[[Image:1zhh.gif|left|200px]]<br /><applet load="1zhh" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1zhh, resolution 1.94&Aring;" />
 
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'''Crystal Structure of the Apo Form of Vibrio Harveyi LUXP Complexed with the Periplasmic Domain of LUXQ'''<br />
 
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==Overview==
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==Crystal Structure of the Apo Form of Vibrio Harveyi LUXP Complexed with the Periplasmic Domain of LUXQ==
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The extracellular signaling molecule autoinducer-2 (AI-2) mediates, quorum-sensing communication in diverse bacterial species. In marine, vibrios, binding of AI-2 to the periplasmic receptor LuxP modulates the, activity of the inner membrane sensor kinase LuxQ, transducing the AI-2, information into the cytoplasm. Here, we show that Vibrio harveyi LuxP, associates with LuxQ in both the presence and absence of AI-2. The 1.9 A, X-ray crystal structure of apoLuxP, complexed with the periplasmic domain, of LuxQ, reveals that the latter contains two tandem, Per/ARNT/Simple-minded (PAS) folds. Thus, although many prokaryotic PAS, folds themselves bind ligands, the LuxQ periplasmic PAS folds instead bind, LuxP, monitoring its AI-2 occupancy. Mutations that disrupt the, apoLuxP:LuxQ interface sensitize V. harveyi to AI-2, implying that AI-2, binding causes the replacement of one set of LuxP:LuxQ contacts with, another. These conformational changes switch LuxQ between two opposing, enzymatic activities, each of which conveys information to the cytoplasm, about the cell density of the surrounding environment.
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<StructureSection load='1zhh' size='340' side='right'caption='[[1zhh]], [[Resolution|resolution]] 1.94&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1zhh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_harveyi Vibrio harveyi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZHH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZHH FirstGlance]. <br>
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1ZHH is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Vibrio_harveyi Vibrio harveyi] with NHE as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ZHH OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.94&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NHE:2-[N-CYCLOHEXYLAMINO]ETHANE+SULFONIC+ACID'>NHE</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zhh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zhh OCA], [https://pdbe.org/1zhh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zhh RCSB], [https://www.ebi.ac.uk/pdbsum/1zhh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zhh ProSAT]</span></td></tr>
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Regulation of LuxPQ receptor activity by the quorum-sensing signal autoinducer-2., Neiditch MB, Federle MJ, Miller ST, Bassler BL, Hughson FM, Mol Cell. 2005 May 27;18(5):507-18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15916958 15916958]
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</table>
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[[Category: Protein complex]]
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== Function ==
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[https://www.uniprot.org/uniprot/LUXP_VIBHA LUXP_VIBHA] Binds to the signaling molecule autoinducer 2 (AI-2), a furanosyl borate diester, (3a-methyl-5,6-dihydrofuro-[2,3d][1,3,2]dioxaborole-2,2,6,6a-tetraol). This complex then interacts with the LuxQ sensor protein.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zh/1zhh_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zhh ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Vibrio harveyi]]
[[Category: Vibrio harveyi]]
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[[Category: Bassler, B.L.]]
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[[Category: Bassler BL]]
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[[Category: Federle, M.J.]]
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[[Category: Federle MJ]]
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[[Category: Hughson, F.M.]]
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[[Category: Hughson FM]]
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[[Category: Miller, S.T.]]
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[[Category: Miller ST]]
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[[Category: Neiditch, M.B.]]
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[[Category: Neiditch MB]]
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[[Category: NHE]]
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[[Category: autoinducer-2 (ai-2)]]
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[[Category: per/arnt/simple-minded (pas) fold]]
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[[Category: periplasmic binding protein]]
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[[Category: quorum sensing]]
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[[Category: sensor kinase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:27:23 2007''
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Current revision

Crystal Structure of the Apo Form of Vibrio Harveyi LUXP Complexed with the Periplasmic Domain of LUXQ

PDB ID 1zhh

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