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1zhi

From Proteopedia

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(New page: 200px<br /><applet load="1zhi" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zhi, resolution 2.70&Aring;" /> '''Complex of the S. ce...)
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[[Image:1zhi.gif|left|200px]]<br /><applet load="1zhi" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1zhi, resolution 2.70&Aring;" />
 
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'''Complex of the S. cerevisiae Orc1 and Sir1 interacting domains'''<br />
 
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==Overview==
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==Complex of the S. cerevisiae Orc1 and Sir1 interacting domains==
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The Sir1 protein plays a key role in establishing a silent chromatin, structure at the cryptic mating-type loci HMR and HML in Saccharomyces, cerevisiae by interacting with the bromo-adjacent homology (BAH) domain of, the Orc1p subunit of the origin recognition complex (ORC). Here, we, present the high-resolution crystal structures of the ORC interaction, region (OIR) of Sir1p and that of the complex formed between the OIR and, BAH domains. Amino acids within the OIR previously shown to be required, for a Sir1p/ORC interaction are presented on a conserved, convex surface, that forms a complementary interface with a concave region of the Orc1 BAH, domain that is critical for transcriptional silencing. The OIR/BAH, interaction surface comprises a network of hydrophobic and polar/ionic, interactions between discrete structural modules in each protein and, involves several residues that were not implicated in previous studies., These data provide important structural insights into a protein-protein, interaction critical for the formation of a specialized chromatin domain, within eukaryotic chromosomes.
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<StructureSection load='1zhi' size='340' side='right'caption='[[1zhi]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1zhi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZHI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZHI FirstGlance]. <br>
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1ZHI is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ZHI OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zhi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zhi OCA], [https://pdbe.org/1zhi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zhi RCSB], [https://www.ebi.ac.uk/pdbsum/1zhi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zhi ProSAT]</span></td></tr>
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==Reference==
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</table>
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Structural basis of the Sir1-origin recognition complex interaction in transcriptional silencing., Hou Z, Bernstein DA, Fox CA, Keck JL, Proc Natl Acad Sci U S A. 2005 Jun 14;102(24):8489-94. Epub 2005 Jun 2. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15932939 15932939]
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== Function ==
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[[Category: Protein complex]]
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[https://www.uniprot.org/uniprot/ORC1_YEAST ORC1_YEAST] Component of the origin recognition complex (ORC) that binds origins of replication. It has a role in both chromosomal replication and mating type transcriptional silencing. Binds to the ARS consensus sequence (ACS) of origins of replication.<ref>PMID:17825064</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zh/1zhi_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zhi ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Bernstein, D.A.]]
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[[Category: Bernstein DA]]
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[[Category: Fox, C.A.]]
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[[Category: Fox CA]]
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[[Category: Hou, Z.]]
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[[Category: Hou Z]]
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[[Category: Keck, J.L.]]
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[[Category: Keck JL]]
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[[Category: bah domain]]
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[[Category: oir domain]]
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[[Category: protein complex]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:27:27 2007''
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Current revision

Complex of the S. cerevisiae Orc1 and Sir1 interacting domains

PDB ID 1zhi

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