2cjk

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{{Seed}}
 
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[[Image:2cjk.png|left|200px]]
 
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==Structure of the RNA binding domain of Hrp1 in complex with RNA==
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The line below this paragraph, containing "STRUCTURE_2cjk", creates the "Structure Box" on the page.
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<StructureSection load='2cjk' size='340' side='right'caption='[[2cjk]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2cjk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CJK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CJK FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cjk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cjk OCA], [https://pdbe.org/2cjk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cjk RCSB], [https://www.ebi.ac.uk/pdbsum/2cjk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cjk ProSAT]</span></td></tr>
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{{STRUCTURE_2cjk| PDB=2cjk | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HRP1_YEAST HRP1_YEAST] RNA-binding protein, which is involved in the polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with the cleavage factor CFIA complex and the cleavage and polyadenylation factor (CPF) complex. May be involved in regulation of poly(A) site selection. Is involved in nonsense-mediated mRNA decay. Seems to bind to an RNA downstream sequence element (DSE) located 3' of a nonsense codon and may mark the transcript for decay.<ref>PMID:9334319</ref> <ref>PMID:9857200</ref> <ref>PMID:10882134</ref> <ref>PMID:11344258</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cj/2cjk_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cjk ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The recognition of specific signals encoded within the 3'-untranslated region of the newly transcribed mRNA triggers the assembly of a multiprotein machine that modifies its 3'-end. Hrp1 recognises one of such signals, the so-called polyadenylation enhancement element (PEE), promoting the recruitment of other polyadenylation factors in yeast. The molecular bases of this interaction are revealed here by the solution structure of a complex between Hrp1 and an oligonucleotide mimicking the PEE. Six consecutive bases (AUAUAU) are specifically recognised by two RNA-binding domains arranged in tandem. Both protein and RNA undergo significant conformational changes upon complex formation with a concomitant large surface burial of RNA bases. Key aspects of RNA specificity can be explained by the presence of intermolecular aromatic-aromatic contacts and hydrogen bonds. Altogether, the Hrp1-PEE structure represents one of the first steps towards understanding of the assembly of the cleavage and polyadenylation machinery at the atomic level.
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===STRUCTURE OF THE RNA BINDING DOMAIN OF HRP1 IN COMPLEX WITH RNA===
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Grabbing the message: structural basis of mRNA 3'UTR recognition by Hrp1.,Perez-Canadillas JM EMBO J. 2006 Jul 12;25(13):3167-78. Epub 2006 Jun 22. PMID:16794580<ref>PMID:16794580</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_16794580}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2cjk" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 16794580 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16794580}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2CJK is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CJK OCA].
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==Reference==
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<ref group="xtra">PMID:16794580</ref><references group="xtra"/>
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[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Perez-Canadillas, J M.]]
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[[Category: Perez-Canadillas JM]]
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[[Category: Cleavage]]
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[[Category: Hrp1]]
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[[Category: Mrna processing]]
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[[Category: Nonsense-mediated mrna decay]]
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[[Category: Nuclear protein]]
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[[Category: Polyadenylation]]
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[[Category: Rna processing]]
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[[Category: Rna-binding]]
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[[Category: Rna-binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 21:23:30 2009''
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Current revision

Structure of the RNA binding domain of Hrp1 in complex with RNA

PDB ID 2cjk

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