1zlq

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(New page: 200px<br /><applet load="1zlq" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zlq, resolution 1.8&Aring;" /> '''Crystallographic and ...)
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[[Image:1zlq.gif|left|200px]]<br /><applet load="1zlq" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1zlq, resolution 1.8&Aring;" />
 
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'''Crystallographic and spectroscopic evidence for high affinity binding of Fe EDTA (H2O)- to the periplasmic nickel transporter NikA'''<br />
 
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==Overview==
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==Crystallographic and spectroscopic evidence for high affinity binding of Fe EDTA (H2O)- to the periplasmic nickel transporter NikA==
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Because nickel is both essential and toxic to a great variety of, organisms, its detection and transport is highly regulated. In Escherichia, coli and other related Gram-negative bacteria, high affinity nickel, transport depends on proteins expressed by the nik operon. A central actor, of this process is the periplasmic NikA transport protein. A previous, structural report has proposed that nickel binds to NikA as a pentahydrate, species. However, both stereochemical considerations and X-ray absorption, spectroscopic results are incompatible with that interpretation. Here, we, report the 1.8 A resolution structure of NikA and show that it binds, FeEDTA(H2O)- with very high affinity. In addition, we provide, crystallographic evidence that a metal-EDTA complex was also bound to the, previously reported NikA structure. Our observations strongly suggest that, nickel transport in E. coli requires the binding of this metal ion to a, metallophore that bears significant resemblance to EDTA. They also provide, a basis for the potential use of NikA in the bioremediation of toxic, transition metals and the design of artificial metalloenzymes.
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<StructureSection load='1zlq' size='340' side='right'caption='[[1zlq]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1zlq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZLQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZLQ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene>, <scene name='pdbligand=EDT:{[-(BIS-CARBOXYMETHYL-AMINO)-ETHYL]-CARBOXYMETHYL-AMINO}-ACETIC+ACID'>EDT</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zlq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zlq OCA], [https://pdbe.org/1zlq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zlq RCSB], [https://www.ebi.ac.uk/pdbsum/1zlq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zlq ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NIKA_ECOLI NIKA_ECOLI] Involved in a nickel transport system, probably represents the nickel binder.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zl/1zlq_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zlq ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Because nickel is both essential and toxic to a great variety of organisms, its detection and transport is highly regulated. In Escherichia coli and other related Gram-negative bacteria, high affinity nickel transport depends on proteins expressed by the nik operon. A central actor of this process is the periplasmic NikA transport protein. A previous structural report has proposed that nickel binds to NikA as a pentahydrate species. However, both stereochemical considerations and X-ray absorption spectroscopic results are incompatible with that interpretation. Here, we report the 1.8 A resolution structure of NikA and show that it binds FeEDTA(H2O)- with very high affinity. In addition, we provide crystallographic evidence that a metal-EDTA complex was also bound to the previously reported NikA structure. Our observations strongly suggest that nickel transport in E. coli requires the binding of this metal ion to a metallophore that bears significant resemblance to EDTA. They also provide a basis for the potential use of NikA in the bioremediation of toxic transition metals and the design of artificial metalloenzymes.
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==About this Structure==
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Crystallographic and spectroscopic evidence for high affinity binding of FeEDTA(H2O)- to the periplasmic nickel transporter NikA.,Cherrier MV, Martin L, Cavazza C, Jacquamet L, Lemaire D, Gaillard J, Fontecilla-Camps JC J Am Chem Soc. 2005 Jul 20;127(28):10075-82. PMID:16011372<ref>PMID:16011372</ref>
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1ZLQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with FE, ACT, SO4, CL, EDT, DTT and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Nickel-transporting_ATPase Nickel-transporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.24 3.6.3.24] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ZLQ OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystallographic and spectroscopic evidence for high affinity binding of FeEDTA(H2O)- to the periplasmic nickel transporter NikA., Cherrier MV, Martin L, Cavazza C, Jacquamet L, Lemaire D, Gaillard J, Fontecilla-Camps JC, J Am Chem Soc. 2005 Jul 20;127(28):10075-82. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16011372 16011372]
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</div>
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[[Category: Escherichia coli]]
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<div class="pdbe-citations 1zlq" style="background-color:#fffaf0;"></div>
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[[Category: Nickel-transporting ATPase]]
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[[Category: Single protein]]
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[[Category: Camps, J.C.Fontecilla.]]
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[[Category: Cavazza, C.]]
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[[Category: Cherrier, M.V.]]
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[[Category: Gaillard, J.]]
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[[Category: Jacquamet, L.]]
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[[Category: Lemaire, D.]]
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[[Category: Martin, L.]]
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[[Category: ACT]]
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[[Category: CL]]
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[[Category: DTT]]
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[[Category: EDT]]
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[[Category: FE]]
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[[Category: GOL]]
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[[Category: SO4]]
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[[Category: bacteria]]
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[[Category: edta]]
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[[Category: nickel]]
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[[Category: periplasm]]
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[[Category: transport]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:31:28 2007''
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==See Also==
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*[[ABC transporter 3D structures|ABC transporter 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Escherichia coli]]
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[[Category: Large Structures]]
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[[Category: Cavazza C]]
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[[Category: Cherrier MV]]
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[[Category: Fontecilla Camps JC]]
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[[Category: Gaillard J]]
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[[Category: Jacquamet L]]
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[[Category: Lemaire D]]
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[[Category: Martin L]]

Current revision

Crystallographic and spectroscopic evidence for high affinity binding of Fe EDTA (H2O)- to the periplasmic nickel transporter NikA

PDB ID 1zlq

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