1r1t

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{{Seed}}
 
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[[Image:1r1t.png|left|200px]]
 
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==Crystal structure of the cyanobacterial metallothionein repressor SmtB in the apo-form==
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The line below this paragraph, containing "STRUCTURE_1r1t", creates the "Structure Box" on the page.
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<StructureSection load='1r1t' size='340' side='right'caption='[[1r1t]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1r1t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechococcus_elongatus_PCC_7942_=_FACHB-805 Synechococcus elongatus PCC 7942 = FACHB-805]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R1T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R1T FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r1t OCA], [https://pdbe.org/1r1t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r1t RCSB], [https://www.ebi.ac.uk/pdbsum/1r1t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r1t ProSAT]</span></td></tr>
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{{STRUCTURE_1r1t| PDB=1r1t | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SMTB_SYNE7 SMTB_SYNE7] Transcriptional repressor of the expression of the smtA gene. Binds two zinc ions per dimer. The complex of DNA and SmtB is dissociated by zinc ions.<ref>PMID:12146943</ref> <ref>PMID:12407207</ref> <ref>PMID:8871549</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r1/1r1t_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r1t ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The origin of metal ion selectivity by members of the SmtB/ArsR family of bacterial metal-sensing transcriptional repressors and the mechanism of negative allosteric regulation of DNA binding is poorly understood. Here, we report that two homologous zinc sensors, Staphylococcus aureus CzrA and cyanobacterial SmtB, are "winged" helix homodimeric DNA-binding proteins that bind Zn(II) to a pair of tetrahedral, interhelical binding sites, with two ligands derived from the alpha5 helix of one subunit, Asp84 O(delta1) (Asp104 in SmtB), His86 N(delta1) (His106), and two derived from the alpha5 helix of the other, His97' N(delta1) (His117') and His100' N(epsilon2) (Glu120'). Formation of the metal chelate drives a quaternary structural switch mediated by an intersubunit hydrogen-binding network that originates with the non-liganding N(epsilon2) face of His97 in CzrA (His117 in SmtB) that stabilizes a low-affinity, DNA-binding conformation. The structure of the Zn(1) SmtB homodimer shows that both metal-binding sites of the dimer must be occupied for the quaternary structural switch to occur. Thus, a critical zinc-ligating histidine residue obligatorily couples formation of the metal-sensing coordination chelate to changes in the conformation and dynamics of the putative DNA-binding helices.
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===Crystal structure of the cyanobacterial metallothionein repressor SmtB in the apo-form===
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A metal-ligand-mediated intersubunit allosteric switch in related SmtB/ArsR zinc sensor proteins.,Eicken C, Pennella MA, Chen X, Koshlap KM, VanZile ML, Sacchettini JC, Giedroc DP J Mol Biol. 2003 Oct 31;333(4):683-95. PMID:14568530<ref>PMID:14568530</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_14568530}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1r1t" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 14568530 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_14568530}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1R1T is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Synechococcus_sp. Synechococcus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R1T OCA].
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[[Category: Synechococcus elongatus PCC 7942 = FACHB-805]]
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[[Category: Chen X]]
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==Reference==
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[[Category: Eicken C]]
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<ref group="xtra">PMID:14568530</ref><references group="xtra"/>
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[[Category: Giedroc DP]]
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[[Category: Synechococcus sp.]]
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[[Category: Koshlap KM]]
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[[Category: Chen, X.]]
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[[Category: Pennella MA]]
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[[Category: Eicken, C.]]
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[[Category: Sacchettini JC]]
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[[Category: Giedroc, D P.]]
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[[Category: VanZile ML]]
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[[Category: Koshlap, K M.]]
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[[Category: Pennella, M A.]]
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[[Category: Sacchettini, J C.]]
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[[Category: VanZile, M L.]]
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[[Category: Dna binding]]
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[[Category: Transcriptional regulation]]
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[[Category: Winged hth protein]]
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[[Category: Zinc]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 21:45:58 2009''
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Current revision

Crystal structure of the cyanobacterial metallothionein repressor SmtB in the apo-form

PDB ID 1r1t

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