1mp1
From Proteopedia
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| - | {{Seed}} | ||
| - | [[Image:1mp1.png|left|200px]] | ||
| - | < | + | ==Solution structure of the PWI motif from SRm160== |
| - | + | <StructureSection load='1mp1' size='340' side='right'caption='[[1mp1]]' scene=''> | |
| - | You may | + | == Structural highlights == |
| - | or the | + | <table><tr><td colspan='2'>[[1mp1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MP1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MP1 FirstGlance]. <br> |
| - | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |
| - | -- | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mp1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mp1 OCA], [https://pdbe.org/1mp1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mp1 RCSB], [https://www.ebi.ac.uk/pdbsum/1mp1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mp1 ProSAT]</span></td></tr> |
| - | + | </table> | |
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/SRRM1_HUMAN SRRM1_HUMAN] Part of pre- and post-splicing multiprotein mRNP complexes. Involved in numerous pre-mRNA processing events. Promotes constitutive and exonic splicing enhancer (ESE)-dependent splicing activation by bridging together sequence-specific (SR family proteins, SFRS4, SFRS5 and TRA2B/SFRS10) and basal snRNP (SNRP70 and SNRPA1) factors of the spliceosome. Stimulates mRNA 3'-end cleavage independently of the formation of an exon junction complex. Binds both pre-mRNA and spliced mRNA 20-25 nt upstream of exon-exon junctions. Binds RNA and DNA with low sequence specificity and has similar preference for either double- or single-stranded nucleic acid substrates.<ref>PMID:9531537</ref> <ref>PMID:10339552</ref> <ref>PMID:10668804</ref> <ref>PMID:11739730</ref> <ref>PMID:12944400</ref> <ref>PMID:12600940</ref> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mp/1mp1_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mp1 ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The PWI motif is a highly conserved domain of unknown function in the SRm160 splicing and 3'-end cleavage-stimulatory factor, as well as in several other known or putative pre-mRNA processing components. We show here that the PWI motif is a new type of RNA/DNA-binding domain that has an equal preference for single- and double-stranded nucleic acids. Deletion of the motif prevents SRm160 from binding RNA and stimulating 3'-end cleavage, and its substitution with a heterologous RNA-binding domain restores these functions. The NMR solution structure of the SRm160-PWI motif reveals a novel, four-helix bundle and represents the first example of an alpha-helical fold that can bind single-stranded (ss)RNA. Structure-guided mutagenesis indicates that the same surface is involved in RNA and DNA binding and requires the cooperative action of a highly conserved, adjacent basic region. Thus, the PWI motif is a novel type of nucleic acid-binding domain that likely has multiple important functions in pre-mRNA processing, including SRm160-dependent stimulation of 3'-end formation. | ||
| - | + | Structure and function of the PWI motif: a novel nucleic acid-binding domain that facilitates pre-mRNA processing.,Szymczyna BR, Bowman J, McCracken S, Pineda-Lucena A, Lu Y, Cox B, Lambermon M, Graveley BR, Arrowsmith CH, Blencowe BJ Genes Dev. 2003 Feb 15;17(4):461-75. PMID:12600940<ref>PMID:12600940</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 1mp1" style="background-color:#fffaf0;"></div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | + | __TOC__ | |
| - | + | </StructureSection> | |
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| - | == | + | |
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
| - | [[Category: Arrowsmith | + | [[Category: Large Structures]] |
| - | [[Category: Blencowe | + | [[Category: Arrowsmith CH]] |
| - | [[Category: Bowman | + | [[Category: Blencowe BJ]] |
| - | [[Category: Cox | + | [[Category: Bowman J]] |
| - | [[Category: Graveley | + | [[Category: Cox B]] |
| - | [[Category: Lambermon | + | [[Category: Graveley BR]] |
| - | [[Category: Lu | + | [[Category: Lambermon M]] |
| - | [[Category: McCracken | + | [[Category: Lu Y]] |
| - | [[Category: Pineda-Lucena | + | [[Category: McCracken S]] |
| - | [[Category: Szymczyna | + | [[Category: Pineda-Lucena A]] |
| - | + | [[Category: Szymczyna BR]] | |
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Current revision
Solution structure of the PWI motif from SRm160
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Categories: Homo sapiens | Large Structures | Arrowsmith CH | Blencowe BJ | Bowman J | Cox B | Graveley BR | Lambermon M | Lu Y | McCracken S | Pineda-Lucena A | Szymczyna BR
