2oca

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{{Seed}}
 
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[[Image:2oca.png|left|200px]]
 
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==The crystal structure of T4 UvsW==
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The line below this paragraph, containing "STRUCTURE_2oca", creates the "Structure Box" on the page.
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<StructureSection load='2oca' size='340' side='right'caption='[[2oca]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2oca]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OCA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OCA FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2oca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oca OCA], [https://pdbe.org/2oca PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2oca RCSB], [https://www.ebi.ac.uk/pdbsum/2oca PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2oca ProSAT]</span></td></tr>
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{{STRUCTURE_2oca| PDB=2oca | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/UVSW_BPT4 UVSW_BPT4] DNA helicase that catalyzes branch migration and dissociation of RNA-DNA hybrids. Plays a critical role in a specific recombination pathway involving simple reciprocal exchange. UvsW protein is normally the key regulatory factor in the switch from early to late phage DNA replication.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oc/2oca_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2oca ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The uvsWXY system is implicated in the replication and repair of the bacteriophage T4 genome. Whereas the roles of the recombinase (UvsX) and the recombination mediator protein (UvsY) are known, the precise role of UvsW is unclear. Sequence analysis identifies UvsW as a member of the monomeric SF2 helicase superfamily that translocates nucleic acid substrates via the action of two RecA-like motor domains. Functional homologies to Escherichia coli RecG and biochemical analyses have shown that UvsW interacts with branched nucleic acid substrates, suggesting roles in recombination and the rescue of stalled replication forks. A sequencing error at the 3'-end of the uvsW gene has revealed a second, short open reading frame that encodes a protein of unknown function called UvsW.1. We have determined the crystal structure of UvsW to 2.7A and the NMR solution structure of UvsW.1. UvsW has a four-domain architecture with structural homology to the eukaryotic SF2 helicase, Rad54. A model of the UvsW-ssDNA complex identifies structural elements and conserved residues that may interact with nucleic acid substrates. The NMR solution structure of UvsW.1 reveals a dynamic four-helix bundle with homology to the structure-specific nucleic acid binding module of RecQ helicases.
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===The crystal structure of T4 UvsW===
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Crystallographic and NMR analyses of UvsW and UvsW.1 from bacteriophage T4.,Kerr ID, Sivakolundu S, Li Z, Buchsbaum JC, Knox LA, Kriwacki R, White SW J Biol Chem. 2007 Nov 23;282(47):34392-400. Epub 2007 Sep 17. PMID:17878153<ref>PMID:17878153</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2oca" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_17878153}}, adds the Publication Abstract to the page
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*[[Helicase 3D structures|Helicase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 17878153 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_17878153}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Escherichia virus T4]]
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2OCA is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t4 Enterobacteria phage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OCA OCA].
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[[Category: Large Structures]]
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[[Category: Kerr ID]]
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==Reference==
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[[Category: White SW]]
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<ref group="xtra">PMID:17878153</ref><references group="xtra"/>
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[[Category: ATP diphosphatase]]
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[[Category: Enterobacteria phage t4]]
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[[Category: Kerr, I D.]]
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[[Category: White, S W.]]
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[[Category: Atp-dependant helicase]]
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[[Category: Hydrolase]]
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[[Category: Recombination]]
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[[Category: T4-bacteriophage]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 22:02:39 2009''
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Current revision

The crystal structure of T4 UvsW

PDB ID 2oca

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OCA

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