1zo1

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(New page: 200px<br /><applet load="1zo1" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zo1" /> '''IF2, IF1, and tRNA fitted to cryo-EM data OF...)
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[[Image:1zo1.gif|left|200px]]<br /><applet load="1zo1" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1zo1" />
 
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'''IF2, IF1, and tRNA fitted to cryo-EM data OF E. COLI 70S initiation complex'''<br />
 
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==Overview==
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==IF2, IF1, and tRNA fitted to cryo-EM data OF E. COLI 70S initiation complex==
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The 70S ribosome and its complement of factors required for initiation of, translation in E. coli were purified separately and reassembled in vitro, with GDPNP, producing a stable initiation complex (IC) stalled after 70S, assembly. We have obtained a cryo-EM reconstruction of the IC showing, IF2*GDPNP at the intersubunit cleft of the 70S ribosome. IF2*GDPNP, contacts the 30S and 50S subunits as well as fMet-tRNA(fMet). IF2 here, adopts a conformation radically different from that seen in the recent, crystal structure of IF2. The C-terminal domain of IF2 binds to the, single-stranded portion of fMet-tRNA(fMet), thereby forcing the tRNA into, a novel orientation at the P site. The GTP binding domain of IF2 binds to, the GTPase-associated center of the 50S subunit in a manner similar to, EF-G and EF-Tu. Additionally, we present evidence for the localization of, IF1, IF3, one C-terminal domain of L7/L12, and the N-terminal domain of, IF2 in the initiation complex.
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<SX load='1zo1' size='340' side='right' viewer='molstar' caption='[[1zo1]], [[Resolution|resolution]] 13.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1zo1]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZO1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZO1 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 13.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zo1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zo1 OCA], [https://pdbe.org/1zo1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zo1 RCSB], [https://www.ebi.ac.uk/pdbsum/1zo1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zo1 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IF2_ECOLI IF2_ECOLI] One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex.[HAMAP-Rule:MF_00100_B]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zo/1zo1_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zo1 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1ZO1 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ZO1 OCA].
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*[[Transfer RNA (tRNA)|Transfer RNA (tRNA)]]
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__TOC__
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==Reference==
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</SX>
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The cryo-EM structure of a translation initiation complex from Escherichia coli., Allen GS, Zavialov A, Gursky R, Ehrenberg M, Frank J, Cell. 2005 Jun 3;121(5):703-12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15935757 15935757]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Allen, G.S.]]
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[[Category: Allen GS]]
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[[Category: Ehrenberg, M.]]
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[[Category: Ehrenberg M]]
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[[Category: Frank, J.]]
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[[Category: Frank J]]
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[[Category: Gursky, R.]]
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[[Category: Gursky R]]
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[[Category: Zavialov, A.]]
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[[Category: Zavialov A]]
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[[Category: cryo-eletron microscopy]]
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[[Category: e. coli]]
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[[Category: initiation factor]]
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[[Category: initiation of protein synthesis]]
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[[Category: ribosome]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:34:03 2007''
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Current revision

IF2, IF1, and tRNA fitted to cryo-EM data OF E. COLI 70S initiation complex

1zo1, resolution 13.80Å

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