2k2f

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (19:09, 29 May 2024) (edit) (undo)
 
(8 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:2k2f.png|left|200px]]
 
-
<!--
+
==Solution structure of Ca2+-S100A1-RyRP12==
-
The line below this paragraph, containing "STRUCTURE_2k2f", creates the "Structure Box" on the page.
+
<StructureSection load='2k2f' size='340' side='right'caption='[[2k2f]]' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2k2f]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K2F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2K2F FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-
{{STRUCTURE_2k2f| PDB=2k2f | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2k2f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k2f OCA], [https://pdbe.org/2k2f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2k2f RCSB], [https://www.ebi.ac.uk/pdbsum/2k2f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2k2f ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/RYR2_RAT RYR2_RAT] Cytosolic calcium-activated calcium channel that mediates the release of Ca(2+) from the sarcoplasmic reticulum into the cytosol and thereby plays a key role in triggering cardiac muscle contraction. Aberrant channel activation can lead to cardiac arrhythmia. In cardiac myocytes, calcium release is triggered by increased Ca(2+) cytosolic levels due to activation of the L-type calcium channel CACNA1C. The calcium channel activity is modulated by formation of heterotetramers with RYR3. Required for cellular calcium ion homeostasis. Required for embryonic heart development.[UniProtKB:Q92736]<ref>PMID:20431056</ref> <ref>PMID:20471962</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k2/2k2f_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2k2f ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
In heart and skeletal muscle an S100 protein family member, S100A1, binds to the ryanodine receptor (RyR) and promotes Ca(2+) release. Using competition binding assays, we further characterized this system in skeletal muscle and showed that Ca(2+)-S100A1 competes with Ca(2+)-calmodulin (CaM) for the same binding site on RyR1. In addition, the NMR structure was determined for Ca(2+)-S100A1 bound to a peptide derived from this CaM/S100A1 binding domain, a region conserved in RyR1 and RyR2 and termed RyRP12 (residues 3616-3627 in human RyR1). Examination of the S100A1-RyRP12 complex revealed residues of the helical RyRP12 peptide (Lys-3616, Trp-3620, Lys-3622, Leu-3623, Leu-3624, and Lys-3626) that are involved in favorable hydrophobic and electrostatic interactions with Ca(2+)-S100A1. These same residues were shown previously to be important for RyR1 binding to Ca(2+)-CaM. A model for regulating muscle contraction is presented in which Ca(2+)-S100A1 and Ca(2+)-CaM compete directly for the same binding site on the ryanodine receptor.
-
===Solution structure of Ca2+-S100A1-RyRP12===
+
S100A1 and calmodulin compete for the same binding site on ryanodine receptor.,Wright NT, Prosser BL, Varney KM, Zimmer DB, Schneider MF, Weber DJ J Biol Chem. 2008 Sep 26;283(39):26676-83. Epub 2008 Jul 23. PMID:18650434<ref>PMID:18650434</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 2k2f" style="background-color:#fffaf0;"></div>
-
<!--
+
==See Also==
-
The line below this paragraph, {{ABSTRACT_PUBMED_18650434}}, adds the Publication Abstract to the page
+
*[[S100 proteins 3D structures|S100 proteins 3D structures]]
-
(as it appears on PubMed at http://www.pubmed.gov), where 18650434 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_18650434}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
[[Category: Large Structures]]
-
2K2F is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K2F OCA].
+
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:18650434</ref><references group="xtra"/>
+
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
-
[[Category: Varney, K M.]]
+
[[Category: Varney KM]]
-
[[Category: Weber, D J.]]
+
[[Category: Weber DJ]]
-
[[Category: Wright, N T.]]
+
[[Category: Wright NT]]
-
[[Category: Alternative splicing]]
+
-
[[Category: Calcium binding]]
+
-
[[Category: Calcium channel]]
+
-
[[Category: Calcium transport]]
+
-
[[Category: Cytoplasm]]
+
-
[[Category: Ef hand]]
+
-
[[Category: Glycoprotein]]
+
-
[[Category: Ion transport]]
+
-
[[Category: Ionic channel]]
+
-
[[Category: Membrane]]
+
-
[[Category: Metal binding protein]]
+
-
[[Category: Metal-binding]]
+
-
[[Category: Polymorphism]]
+
-
[[Category: Ryanodine receptor]]
+
-
[[Category: S100]]
+
-
[[Category: Transmembrane]]
+
-
[[Category: Transport]]
+
-
[[Category: Zinc]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 22:10:47 2009''
+

Current revision

Solution structure of Ca2+-S100A1-RyRP12

PDB ID 2k2f

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2k2f"
Personal tools