1zp7

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(New page: 200px<br /><applet load="1zp7" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zp7, resolution 2.25&Aring;" /> '''The structure of Bac...)
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[[Image:1zp7.gif|left|200px]]<br /><applet load="1zp7" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1zp7, resolution 2.25&Aring;" />
 
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'''The structure of Bacillus subtilis RecU Holliday junction resolvase and its role in substrate selection and sequence specific cleavage.'''<br />
 
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==Overview==
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==The structure of Bacillus subtilis RecU Holliday junction resolvase and its role in substrate selection and sequence specific cleavage.==
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We have determined the structure of the enzyme RecU from Bacillus, subtilis, that is the general Holliday junction resolving enzyme in, Gram-positive bacteria. The enzyme fold reveals a striking similarity to a, class of resolvase enzymes found in archaeal sources and members of the, type II restriction endonuclease family to which they are related. The, structure confirms the presence of active sites formed around clusters of, acidic residues that we have also shown to bind divalent cations., Mutagenesis data presented here support the key role of certain residues., The RecU structure suggests a basis for Holliday junction selectivity and, suggests how sequence-specific cleavage might be achieved. Models for a, resolvase-DNA complex address how the enzyme might organize junctions into, an approximately 4-fold symmetric form.
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<StructureSection load='1zp7' size='340' side='right'caption='[[1zp7]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1zp7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZP7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZP7 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zp7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zp7 OCA], [https://pdbe.org/1zp7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zp7 RCSB], [https://www.ebi.ac.uk/pdbsum/1zp7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zp7 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RECU_BACSU RECU_BACSU] Has at least 2 separable functions; Holliday junction resolution with generation of monomeric chromosomes, and modulation of RecA activity. Endonuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves mobile four-strand junctions by introducing symmetrical nicks in paired strands. Promotes annealing of linear ssDNA with homologous dsDNA. Required for DNA repair, homologous recombination and chromosome segregation. Partially inhibits the hydrolysis of dATP or rATP by RecA. Holliday junction resolution is stimulated by RuvB.<ref>PMID:14701911</ref> <ref>PMID:16020779</ref> <ref>PMID:16154091</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zp/1zp7_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zp7 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1ZP7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ZP7 OCA].
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*[[Resolvase 3D structures|Resolvase 3D structures]]
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== References ==
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==Reference==
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<references/>
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The structure of Bacillus subtilis RecU Holliday junction resolvase and its role in substrate selection and sequence-specific cleavage., McGregor N, Ayora S, Sedelnikova S, Carrasco B, Alonso JC, Thaw P, Rafferty J, Structure. 2005 Sep;13(9):1341-51. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16154091 16154091]
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__TOC__
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</StructureSection>
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Alonso, J.C.]]
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[[Category: Alonso JC]]
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[[Category: Ayora, S.]]
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[[Category: Ayora S]]
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[[Category: Carrasco, B.]]
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[[Category: Carrasco B]]
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[[Category: McGregor, N.]]
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[[Category: McGregor N]]
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[[Category: Rafferty, J.]]
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[[Category: Rafferty J]]
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[[Category: Sedelnikova, S.]]
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[[Category: Sedelnikova S]]
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[[Category: Thaw, P.]]
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[[Category: Thaw P]]
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[[Category: dna-binding protein]]
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[[Category: recombination]]
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[[Category: resolvase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:35:11 2007''
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Current revision

The structure of Bacillus subtilis RecU Holliday junction resolvase and its role in substrate selection and sequence specific cleavage.

PDB ID 1zp7

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