2sxl

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[[Image:2sxl.png|left|200px]]
 
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==SEX-LETHAL RBD1, NMR, MINIMIZED AVERAGE STRUCTURE==
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The line below this paragraph, containing "STRUCTURE_2sxl", creates the "Structure Box" on the page.
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<StructureSection load='2sxl' size='340' side='right'caption='[[2sxl]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2sxl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2SXL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2SXL FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2sxl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2sxl OCA], [https://pdbe.org/2sxl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2sxl RCSB], [https://www.ebi.ac.uk/pdbsum/2sxl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2sxl ProSAT], [https://www.topsan.org/Proteins/RSGI/2sxl TOPSAN]</span></td></tr>
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{{STRUCTURE_2sxl| PDB=2sxl | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SXL_DROME SXL_DROME] Sex determination switch protein which controls sexual development by sex-specific splicing. Regulates dosage compensation in females by suppressing hyperactivation of X-linked genes. Expression of the embryo-specific isoform is under the control of primary sex-determining signal, which depends on the ratio of X chromosomes relative to autosomes (X:A ratio). Expression occurs in 2X:2A cells, but not in X:2A cells. The X:A ratio seems to be signaled by the relative concentration of the X-linked transcription factors SIS-A and SIS-B. As a result, the embryo-specific product is expressed early only in female embryos and specifies female-adult specific splicing; in the male where it is not expressed, the default splicing gives rise to a truncated non-functional protein. The female-specific isoform specifies the splicing of its own transcript, thereby initiating a positive autoregulatory feedback loop leading to female development pathway. The female-specific isoform controls the sex-specific splicing of transformer (TRA); acts as a translational repressor for male-specific lethal-2 (MSL-2) and prevents male-less (MLE), MSL-1 and MSL-3 proteins from associating with the female X chromosome.<ref>PMID:3144435</ref> <ref>PMID:1710769</ref> <ref>PMID:1547493</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sx/2sxl_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2sxl ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The Sex-lethal (Sxl) protein from Drosophila melanogaster has two RNA-binding domains (RBDs). As the amino-terminal RBD (RBD1) of the Sxl protein exhibits low sequence homology to the typical RBDs, particularly at the putative functional residues, it was difficult to unambiguously locate the RNP1 and RNP2 motifs. Therefore, in the present study, we defined the amino and carboxy-terminal borders of the first RNA-binding domain (RBD1) of the Sxl protein by limited tryptic digestion. By replacement of Phe166 by Tyr, we constructed a highly soluble mutant, which exhibits the same RNA-binding properties as those of the wild-type. Using this mutant protein, we performed NMR measurements, and elucidated the secondary and tertiary structures of the Sxl RBD1 in solution. The betaalphabetabetaalphabeta folding pattern is conserved in the solution structure of the Sxl RBD1, as in other reported RBD structures. This allowed us to identify both the RNP1 and RNP2 motifs of the Sxl RBD1 unambiguously. Intriguingly, the RNP2 motif of the Sxl RBD1 has an Ile residue at the second position, which is generally occupied by an aromatic amino acid residue in RBDs and has been suggested to be involved in their RNA binding. Furthermore, the loop region between beta2 and beta3 of the Sxl RBD1 has an exceptional cluster of aromatic amino acid residues, in place of the normal basic amino acid cluster. In contrast, the second RBD of Sxl does not exhibit these characteristic features.
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===SEX-LETHAL RBD1, NMR, MINIMIZED AVERAGE STRUCTURE===
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A characteristic arrangement of aromatic amino acid residues in the solution structure of the amino-terminal RNA-binding domain of Drosophila sex-lethal.,Inoue M, Muto Y, Sakamoto H, Kigawa T, Takio K, Shimura Y, Yokoyama S J Mol Biol. 1997 Sep 12;272(1):82-94. PMID:9299339<ref>PMID:9299339</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_9299339}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2sxl" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 9299339 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_9299339}}
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__TOC__
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</StructureSection>
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==About this Structure==
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2SXL is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2SXL OCA].
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==Reference==
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<ref group="xtra">PMID:9299339</ref><references group="xtra"/>
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[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
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[[Category: Inoue, M.]]
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[[Category: Large Structures]]
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[[Category: Kigawa, T.]]
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[[Category: Inoue M]]
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[[Category: Muto, Y.]]
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[[Category: Kigawa T]]
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[[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]]
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[[Category: Muto Y]]
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[[Category: Sakamoto, H.]]
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[[Category: Sakamoto H]]
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[[Category: Shimura, Y.]]
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[[Category: Shimura Y]]
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[[Category: Takio, K.]]
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[[Category: Takio K]]
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[[Category: Yokoyama, S.]]
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[[Category: Yokoyama S]]
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[[Category: Alternative splicing]]
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[[Category: Riken structural genomics/proteomics initiative]]
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[[Category: Rna-binding domain]]
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[[Category: Rsgi]]
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[[Category: Structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 22:51:26 2009''
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Current revision

SEX-LETHAL RBD1, NMR, MINIMIZED AVERAGE STRUCTURE

PDB ID 2sxl

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