1oln

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{{Seed}}
 
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[[Image:1oln.png|left|200px]]
 
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==Model for thiostrepton antibiotic binding to L11 substrate from 50S ribosomal RNA==
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The line below this paragraph, containing "STRUCTURE_1oln", creates the "Structure Box" on the page.
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<StructureSection load='1oln' size='340' side='right'caption='[[1oln]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1oln]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_azureus Streptomyces azureus] and [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OLN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OLN FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Hybrid , Solution NMR , Theoretical Model, 1 model</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BB9:(2Z)-2-AMINO-3-SULFANYLPROP-2-ENOIC+ACID'>BB9</scene>, <scene name='pdbligand=DBU:(2Z)-2-AMINOBUT-2-ENOIC+ACID'>DBU</scene>, <scene name='pdbligand=DCY:D-CYSTEINE'>DCY</scene>, <scene name='pdbligand=DHA:2-AMINO-ACRYLIC+ACID'>DHA</scene>, <scene name='pdbligand=MH6:3-HYDROXY-2-IMINOPROPANOIC+ACID'>MH6</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=QUA:8-HYDROXY-4-(1-HYDROXYETHYL)QUINOLINE-2-CARBOXYLIC+ACID'>QUA</scene>, <scene name='pdbligand=TS9:(2S,3S,4R)-2-AMINO-3,4-DIHYDROXY-3-METHYLPENTANOIC+ACID'>TS9</scene></td></tr>
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{{STRUCTURE_1oln| PDB=1oln | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1oln FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oln OCA], [https://pdbe.org/1oln PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1oln RCSB], [https://www.ebi.ac.uk/pdbsum/1oln PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1oln ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RL11_THEMA RL11_THEMA] This protein binds directly to 23S ribosomal RNA.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ol/1oln_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1oln ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Thiostrepton and micrococcin inhibit protein synthesis by binding to the L11 binding domain (L11BD) of 23S ribosomal RNA. The two compounds are structurally related, yet they produce different effects on ribosomal RNA in footprinting experiments and on elongation factor-G (EF-G)-dependent GTP hydrolysis. Using NMR and an assay based on A1067 methylation by thiostrepton-resistance methyltransferase, we show that the related thiazoles, nosiheptide and siomycin, also bind to this region. The effect of all four antibiotics on EF-G-dependent GTP hydrolysis and EF-G-GDP-ribosome complex formation was studied. Our NMR and biochemical data demonstrate that thiostrepton, nosiheptide, and siomycin share a common profile, which differs from that of micrococcin. We have generated a three-dimensional (3D) model for the interaction of thiostrepton with L11BD RNA. The model rationalizes the differences between micrococcin and the thiostrepton-like antibiotics interacting with L11BD.
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===MODEL FOR THIOSTREPTON ANTIBIOTIC BINDING TO L11 SUBSTRATE FROM 50S RIBOSOMAL RNA===
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Structural basis for contrasting activities of ribosome binding thiazole antibiotics.,Lentzen G, Klinck R, Matassova N, Aboul-ela F, Murchie AI Chem Biol. 2003 Aug;10(8):769-78. PMID:12954336<ref>PMID:12954336</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1oln" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_12954336}}, adds the Publication Abstract to the page
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*[[Ribosomal protein L11 3D structures|Ribosomal protein L11 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 12954336 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_12954336}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1OLN is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OLN OCA].
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[[Category: Streptomyces azureus]]
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==Reference==
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<ref group="xtra">PMID:12954336</ref><references group="xtra"/>
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[[Category: Thermotoga maritima]]
[[Category: Thermotoga maritima]]
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[[Category: Aboul-Ela, F.]]
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[[Category: Aboul-Ela F]]
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[[Category: Klinck, R.]]
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[[Category: Klinck R]]
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[[Category: Lentzen, G.]]
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[[Category: Lentzen G]]
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[[Category: Matassova, N.]]
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[[Category: Matassova N]]
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[[Category: Murchie, A I.H.]]
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[[Category: Murchie AIH]]
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[[Category: 50]]
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[[Category: Antibiotic]]
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[[Category: L11]]
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[[Category: Ribosome]]
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[[Category: Rna]]
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[[Category: Thiazole]]
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[[Category: Thiostrepton]]
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[[Category: Translation inhibition]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 22:53:12 2009''
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Current revision

Model for thiostrepton antibiotic binding to L11 substrate from 50S ribosomal RNA

PDB ID 1oln

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