2z9o

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{{Seed}}
 
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[[Image:2z9o.png|left|200px]]
 
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==Crystal structure of the dimeric form of RepE in complex with the repE operator DNA==
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The line below this paragraph, containing "STRUCTURE_2z9o", creates the "Structure Box" on the page.
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<StructureSection load='2z9o' size='340' side='right'caption='[[2z9o]], [[Resolution|resolution]] 3.14&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2z9o]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z9O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Z9O FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.14&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2z9o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z9o OCA], [https://pdbe.org/2z9o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2z9o RCSB], [https://www.ebi.ac.uk/pdbsum/2z9o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2z9o ProSAT]</span></td></tr>
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{{STRUCTURE_2z9o| PDB=2z9o | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/REPE1_ECOLI REPE1_ECOLI] Replication initiator in the monomeric form, and autogenous repressor in the dimeric form.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z9/2z9o_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2z9o ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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DNA replication initiator protein RepE stringently regulates F plasmid replication by its two distinct molecular association states. A predominant dimer functions as an autogenous repressor, whereas monomers act as replication initiators, and the dimer requires actions of the DnaK molecular chaperone system for monomerization. The structure of the monomeric form is known, whereas the dimeric structure and structural details of the dimer-to-monomer conversion have been unclear. Here we present the crystal structure of the RepE dimer in complex with the repE operator DNA. The dimerization interface is mainly formed by intermolecular beta-sheets with several key interactions of charged residues. The conformations of the internal N- and C-terminal domains are conserved between the dimer and monomer, whereas the relative domain orientations are strikingly different, allowing for an efficient oligomeric transition of dual-functional RepE. This domain relocation accompanies secondary structural changes in the linker connecting the two domains, and the linker is included in plausible DnaK/DnaJ-binding regions. These findings suggest an activation mechanism for F plasmid replication by RepE monomerization, which is induced and mediated by the DnaK system.
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===Crystal structure of the dimeric form of RepE in complex with the repE operator DNA===
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Structural basis for regulation of bifunctional roles in replication initiator protein.,Nakamura A, Wada C, Miki K Proc Natl Acad Sci U S A. 2007 Nov 20;104(47):18484-9. Epub 2007 Nov 13. PMID:18000058<ref>PMID:18000058</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_18000058}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2z9o" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 18000058 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18000058}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Escherichia coli K-12]]
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2Z9O is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z9O OCA].
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[[Category: Large Structures]]
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[[Category: Miki K]]
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==Reference==
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[[Category: Nakamura A]]
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<ref group="xtra">PMID:18000058</ref><references group="xtra"/>
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[[Category: Wada C]]
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[[Category: Escherichia coli]]
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[[Category: Miki, K.]]
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[[Category: Nakamura, A.]]
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[[Category: Wada, C.]]
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[[Category: Autogenous repressor]]
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[[Category: Dna replication]]
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[[Category: Dna-binding]]
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[[Category: Plasmid]]
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[[Category: Plasmid copy control]]
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[[Category: Protein-dna complex]]
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[[Category: Replication initiator]]
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[[Category: Replication/dna complex]]
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[[Category: Winged helix-turn-helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 22:53:28 2009''
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Current revision

Crystal structure of the dimeric form of RepE in complex with the repE operator DNA

PDB ID 2z9o

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