3psg

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{{Seed}}
 
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[[Image:3psg.png|left|200px]]
 
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==THE HIGH RESOLUTION CRYSTAL STRUCTURE OF PORCINE PEPSINOGEN==
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The line below this paragraph, containing "STRUCTURE_3psg", creates the "Structure Box" on the page.
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<StructureSection load='3psg' size='340' side='right'caption='[[3psg]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3psg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1psg 1psg]. The December 2000 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Pepsin'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2000_12 10.2210/rcsb_pdb/mom_2000_12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PSG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PSG FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3psg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3psg OCA], [https://pdbe.org/3psg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3psg RCSB], [https://www.ebi.ac.uk/pdbsum/3psg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3psg ProSAT]</span></td></tr>
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{{STRUCTURE_3psg| PDB=3psg | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PEPA_PIG PEPA_PIG] Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ps/3psg_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3psg ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The structure of porcine pepsinogen at pH 6.1 has been refined to an R-factor of 0.173 for data extending to 1.65 A. The final model contains 180 solvent molecules and lacks density for residues 157-161. The structure of this aspartic proteinase zymogen possesses many of the characteristics of pepsin, the mature enzyme. The secondary structure of the zymogen consists predominantly of beta-sheet, with an approximate 2-fold axis of symmetry. The activation peptide packs into the active site cleft, and the N-terminus (1P-9P) occupies the position of the mature N-terminus (1-9). Thus changes upon activation include excision of the activation peptide and proper relocation of the mature N-terminus. The activation peptide or residues of the displaced mature N-terminus make specific interactions with the substrate binding subsites. The active site of pepsinogen is intact; thus the lack of activity of pepsinogen is not due to a deformation of the active site. Nine ion pairs in pepsinogen may be important in the advent of activation and involve the activation peptide or regions of the mature N-terminus which are relocated in the mature enzyme. The activation peptide-pepsin junction, 44P-1, is characterized by high thermal parameters and weak density, indicating a flexible structure which would be accessible to cleavage. Pepsinogen is an appropriate model for the structures of other zymogens in the aspartic proteinase family.
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===THE HIGH RESOLUTION CRYSTAL STRUCTURE OF PORCINE PEPSINOGEN===
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The high-resolution crystal structure of porcine pepsinogen.,Hartsuck JA, Koelsch G, Remington SJ Proteins. 1992 May;13(1):1-25. PMID:1594574<ref>PMID:1594574</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3psg" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_1594574}}, adds the Publication Abstract to the page
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*[[Pepsin|Pepsin]]
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(as it appears on PubMed at http://www.pubmed.gov), where 1594574 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_1594574}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3PSG is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1psg 1psg]. The December 2000 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Pepsin'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2000_12 10.2210/rcsb_pdb/mom_2000_12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PSG OCA].
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==Reference==
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<ref group="xtra">PMID:1594574</ref><references group="xtra"/>
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[[Category: Pepsin]]
[[Category: Pepsin]]
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[[Category: RCSB PDB Molecule of the Month]]
[[Category: Sus scrofa]]
[[Category: Sus scrofa]]
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[[Category: Hartsuck, J A.]]
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[[Category: Hartsuck JA]]
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[[Category: Koelsch, G.]]
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[[Category: Koelsch G]]
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[[Category: Remington, S J.]]
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[[Category: Remington SJ]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 22:58:01 2009''
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Current revision

THE HIGH RESOLUTION CRYSTAL STRUCTURE OF PORCINE PEPSINOGEN

PDB ID 3psg

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