2ci5

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /> <applet load="2ci5" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ci5, resolution 1.79&Aring;" /> '''CRYSTAL STRUCTURE O...)
Current revision (12:14, 6 March 2024) (edit) (undo)
 
(22 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2ci5.gif|left|200px]]<br />
 
-
<applet load="2ci5" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="2ci5, resolution 1.79&Aring;" />
 
-
'''CRYSTAL STRUCTURE OF DIMETHYLARGININE DIMETHYLAMINOHYDROLASE I IN COMPLEX WITH L-HOMOCYSTEINE'''<br />
 
-
==Overview==
+
==Crystal structure of Dimethylarginine Dimethylaminohydrolase I in complex with L-homocysteine==
-
Dimethylarginine dimethylaminohydrolase (DDAH) is involved in the, regulation of nitric oxide synthase (NOS) by metabolizing the free, endogenous arginine derivatives N(omega)-methyl-L-arginine (MMA) and, N(omega),N(omega)-dimethyl-L-arginine (ADMA), which are competitive, inhibitors of NOS. Here, we present high-resolution crystal structures of, DDAH isoform 1 (DDAH-1) isolated from bovine brain in complex with, different inhibitors, including S-nitroso-L-homocysteine and Zn2+, a, regulator of this mammalian enzyme. The structure of DDAH-1 consists of a, propeller-like fold similar to other arginine-modifying enzymes and a, flexible loop, which adopts different conformations and acts as a lid at, the entrance of the active site. The orientation and interaction mode of, inhibitors in the ... [[http://ispc.weizmann.ac.il/pmbin/getpm?16698551 (full description)]]
+
<StructureSection load='2ci5' size='340' side='right'caption='[[2ci5]], [[Resolution|resolution]] 1.79&Aring;' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[2ci5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CI5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CI5 FirstGlance]. <br>
-
2CI5 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]] with HCS and CIT as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.3.18 3.5.3.18]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CI5 OCA]].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.79&#8491;</td></tr>
-
 
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=HCS:2-AMINO-4-MERCAPTO-BUTYRIC+ACID'>HCS</scene></td></tr>
-
==Reference==
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ci5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ci5 OCA], [https://pdbe.org/2ci5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ci5 RCSB], [https://www.ebi.ac.uk/pdbsum/2ci5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ci5 ProSAT]</span></td></tr>
-
Structure of the mammalian NOS regulator dimethylarginine dimethylaminohydrolase: A basis for the design of specific inhibitors., Frey D, Braun O, Briand C, Vasak M, Grutter MG, Structure. 2006 May;14(5):901-11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16698551 16698551]
+
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/DDAH1_BOVIN DDAH1_BOVIN] Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ci/2ci5_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ci5 ConSurf].
 +
<div style="clear:both"></div>
 +
__TOC__
 +
</StructureSection>
[[Category: Bos taurus]]
[[Category: Bos taurus]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Braun, O.]]
+
[[Category: Braun O]]
-
[[Category: Briand, C.]]
+
[[Category: Briand C]]
-
[[Category: Frey, D.]]
+
[[Category: Frey D]]
-
[[Category: Grutter, M.G.]]
+
[[Category: Grutter MG]]
-
[[Category: Vasak, M.]]
+
[[Category: Vasak M]]
-
[[Category: CIT]]
+
-
[[Category: HCS]]
+
-
[[Category: acetylation]]
+
-
[[Category: adma]]
+
-
[[Category: hydrolase]]
+
-
[[Category: metal-binding]]
+
-
[[Category: mma]]
+
-
[[Category: no]]
+
-
[[Category: nos regulation]]
+
-
[[Category: s-nitrosylation]]
+
-
[[Category: zinc]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 21:40:09 2007''
+

Current revision

Crystal structure of Dimethylarginine Dimethylaminohydrolase I in complex with L-homocysteine

PDB ID 2ci5

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ci5"
Personal tools