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2b9b

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{{Seed}}
 
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[[Image:2b9b.png|left|200px]]
 
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==Structure of the Parainfluenza Virus 5 F Protein in its Metastable, Pre-fusion Conformation==
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The line below this paragraph, containing "STRUCTURE_2b9b", creates the "Structure Box" on the page.
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<StructureSection load='2b9b' size='340' side='right'caption='[[2b9b]], [[Resolution|resolution]] 2.85&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2b9b]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mammalian_orthorubulavirus_5 Mammalian orthorubulavirus 5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B9B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B9B FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.85&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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{{STRUCTURE_2b9b| PDB=2b9b | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b9b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b9b OCA], [https://pdbe.org/2b9b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b9b RCSB], [https://www.ebi.ac.uk/pdbsum/2b9b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b9b ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FUS_PIV5 FUS_PIV5] Class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and plasma cell membrane fusion, the heptad repeat (HR) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and plasma cell membranes. Directs fusion of viral and cellular membranes leading to delivery of the nucleocapsid into the cytoplasm. This fusion is pH independent and occurs directly at the outer cell membrane. The trimer of F1-F2 (F protein) probably interacts with HN at the virion surface. Upon HN binding to its cellular receptor, the hydrophobic fusion peptide is unmasked and interacts with the cellular membrane, inducing the fusion between cell and virion membranes. Later in infection, F proteins expressed at the plasma membrane of infected cells could mediate fusion with adjacent cells to form syncytia, a cytopathic effect that could lead to tissue necrosis (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b9/2b9b_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2b9b ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Enveloped viruses have evolved complex glycoprotein machinery that drives the fusion of viral and cellular membranes, permitting entry of the viral genome into the cell. For the paramyxoviruses, the fusion (F) protein catalyses this membrane merger and entry step, and it has been postulated that the F protein undergoes complex refolding during this process. Here we report the crystal structure of the parainfluenza virus 5 F protein in its prefusion conformation, stabilized by the addition of a carboxy-terminal trimerization domain. The structure of the F protein shows that there are profound conformational differences between the pre- and postfusion states, involving transformations in secondary and tertiary structure. The positions and structural transitions of key parts of the fusion machinery, including the hydrophobic fusion peptide and two helical heptad repeat regions, clarify the mechanism of membrane fusion mediated by the F protein.
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===Structure of the Parainfluenza Virus 5 F Protein in its Metastable, Pre-fusion Conformation===
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Structure of the parainfluenza virus 5 F protein in its metastable, prefusion conformation.,Yin HS, Wen X, Paterson RG, Lamb RA, Jardetzky TS Nature. 2006 Jan 5;439(7072):38-44. PMID:16397490<ref>PMID:16397490</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 2b9b" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 16397490 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16397490}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2B9B is a 3 chains structure of sequences from [http://en.wikipedia.org/wiki/Simian_virus_5 Simian virus 5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B9B OCA].
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[[Category: Mammalian orthorubulavirus 5]]
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[[Category: Jardetzky TS]]
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==Reference==
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[[Category: Lamb RA]]
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<ref group="xtra">PMID:16397490</ref><references group="xtra"/>
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[[Category: Paterson RG]]
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[[Category: Simian virus 5]]
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[[Category: Wen X]]
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[[Category: Jardetzky, T S.]]
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[[Category: Yin H-S]]
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[[Category: Lamb, R A.]]
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[[Category: Paterson, R G.]]
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[[Category: Wen, X.]]
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[[Category: Yin, H S.]]
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[[Category: Fusion protein]]
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[[Category: Pre-fusion conformation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 23:08:46 2009''
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Current revision

Structure of the Parainfluenza Virus 5 F Protein in its Metastable, Pre-fusion Conformation

PDB ID 2b9b

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