1zuw

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(New page: 200px<br /><applet load="1zuw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zuw, resolution 1.75&Aring;" /> '''Crystal structure of...)
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[[Image:1zuw.gif|left|200px]]<br /><applet load="1zuw" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1zuw, resolution 1.75&Aring;" />
 
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'''Crystal structure of B.subtilis glutamate racemase (RacE) with D-Glu'''<br />
 
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==Overview==
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==Crystal structure of B.subtilis glutamate racemase (RacE) with D-Glu==
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D-glutamate is an essential building block of the peptidoglycan layer in, bacterial cell walls and can be synthesized from L-glutamate by glutamate, racemase (RacE). The structure of a complex of B. subtilis RacE with, D-glutamate reveals that the glutamate is buried in a deep pocket, whose, formation at the interface of the enzyme's two domains involves a, large-scale conformational rearrangement. These domains are related by, pseudo-2-fold symmetry, which superimposes the two catalytic cysteine, residues, which are located at equivalent positions on either side of the, alpha carbon of the substrate. The structural similarity of these two, domains suggests that the racemase activity of RacE arose as a result of, gene duplication. The structure of the complex is dramatically different, from that proposed previously and provides new insights into the RacE, mechanism and an explanation for the potency of a family of RacE, inhibitors, which have been developed as novel antibiotics.
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<StructureSection load='1zuw' size='340' side='right'caption='[[1zuw]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1zuw]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZUW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZUW FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DGL:D-GLUTAMIC+ACID'>DGL</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zuw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zuw OCA], [https://pdbe.org/1zuw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zuw RCSB], [https://www.ebi.ac.uk/pdbsum/1zuw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zuw ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MURI1_BACSU MURI1_BACSU] Provides the (R)-glutamate required for cell wall biosynthesis (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zu/1zuw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zuw ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1ZUW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with DGL as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Glutamate_racemase Glutamate racemase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.1.3 5.1.1.3] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ZUW OCA].
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*[[Glutamate racemase 3D structures|Glutamate racemase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Substrate-induced conformational changes in Bacillus subtilis glutamate racemase and their implications for drug discovery., Ruzheinikov SN, Taal MA, Sedelnikova SE, Baker PJ, Rice DW, Structure. 2005 Nov;13(11):1707-13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16271894 16271894]
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[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: Glutamate racemase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Baker PJ]]
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[[Category: Baker, P.J.]]
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[[Category: Rice DW]]
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[[Category: Rice, D.W.]]
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[[Category: Ruzheinikov SN]]
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[[Category: Ruzheinikov, S.N.]]
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[[Category: Sedelnikova SE]]
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[[Category: Sedelnikova, S.E.]]
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[[Category: Taal MA]]
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[[Category: Taal, M.A.]]
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[[Category: DGL]]
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[[Category: glutamate racemase; (r)-glutamate; peptidoglycan biosynthesis]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:40:38 2007''
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Current revision

Crystal structure of B.subtilis glutamate racemase (RacE) with D-Glu

PDB ID 1zuw

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