2p9h

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{{Seed}}
 
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[[Image:2p9h.png|left|200px]]
 
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==High resolution structure of the Lactose Repressor bound to IPTG==
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The line below this paragraph, containing "STRUCTURE_2p9h", creates the "Structure Box" on the page.
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<StructureSection load='2p9h' size='340' side='right'caption='[[2p9h]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2p9h]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P9H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2P9H FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IPT:ISOPROPYL-1-BETA-D-THIOGALACTOSIDE'>IPT</scene></td></tr>
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{{STRUCTURE_2p9h| PDB=2p9h | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2p9h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p9h OCA], [https://pdbe.org/2p9h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2p9h RCSB], [https://www.ebi.ac.uk/pdbsum/2p9h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2p9h ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LACI_ECOLI LACI_ECOLI] Repressor of the lactose operon. Binds allolactose as an inducer.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p9/2p9h_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2p9h ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The lac operon is a model system for understanding how effector molecules regulate transcription and are necessary for allosteric transitions. The crystal structures of the lac repressor bound to inducer and anti-inducer molecules provide a model for how these small molecules can modulate repressor function. The structures of the apo repressor and the repressor bound to effector molecules are compared in atomic detail. All effectors examined here bind to the repressor in the same location and are anchored to the repressor through hydrogen bonds to several hydroxyl groups of the sugar ring. Inducer molecules form a more extensive hydrogen-bonding network compared to anti-inducers and neutral effector molecules. The structures of these effector molecules suggest that the O6 hydroxyl on the galactoside is essential for establishing a water-mediated hydrogen bonding network that bridges the N-terminal and C-terminal sub-domains. The altered hydrogen bonding can account in part for the different structural conformations of the repressor, and is vital for the allosteric transition.
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===High resolution structure of the Lactose Repressor bound to IPTG===
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Structural analysis of lac repressor bound to allosteric effectors.,Daber R, Stayrook S, Rosenberg A, Lewis M J Mol Biol. 2007 Jul 20;370(4):609-19. Epub 2007 Apr 19. PMID:17543986<ref>PMID:17543986</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2p9h" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_17543986}}, adds the Publication Abstract to the page
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*[[Lac repressor|Lac repressor]]
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(as it appears on PubMed at http://www.pubmed.gov), where 17543986 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_17543986}}
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__TOC__
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</StructureSection>
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==About this Structure==
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2P9H is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P9H OCA].
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==Reference==
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<ref group="xtra">PMID:17543986</ref><references group="xtra"/>
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Daber, R.]]
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[[Category: Large Structures]]
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[[Category: Allosteric effector]]
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[[Category: Daber R]]
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[[Category: Gene regulation]]
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[[Category: Lac repressor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 23:47:06 2009''
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Current revision

High resolution structure of the Lactose Repressor bound to IPTG

PDB ID 2p9h

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