1y97

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[[Image:1y97.png|left|200px]]
 
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==The human TREX2 3' exonuclease structure suggests a mechanism for efficient non-processive DNA catalysis==
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The line below this paragraph, containing "STRUCTURE_1y97", creates the "Structure Box" on the page.
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<StructureSection load='1y97' size='340' side='right'caption='[[1y97]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1y97]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y97 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Y97 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_1y97| PDB=1y97 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1y97 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y97 OCA], [https://pdbe.org/1y97 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1y97 RCSB], [https://www.ebi.ac.uk/pdbsum/1y97 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1y97 ProSAT]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/y9/1y97_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1y97 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The 3' --&gt; 5'-exonucleases process DNA ends in many DNA repair pathways of human cells. Determination of the human TREX2 structure is the first of a dimeric 3'-deoxyribonuclease and indicates how this highly efficient nonprocessive enzyme removes nucleotides at DNA 3' termini. Symmetry in the TREX2 dimer positions the active sites at opposite outer edges providing open access for the DNA. Adjacent to each active site is a flexible region containing three arginines positioned appropriately to bind DNA and to control its entry into the active site. Mutation of these three arginines to alanines reduces the DNA binding capacity by approximately 100-fold with no effect on catalysis. The human TREX2 catalytic residues overlay with the bacterial DnaQ family of 3'-exonucleases confirming the structural conservation of the catalytic sites despite limited sequence identity, and mutations of these residues decrease the still measurable activity by approximately 10(5)-fold, confirming their catalytic role.
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===The human TREX2 3' exonuclease structure suggests a mechanism for efficient non-processive DNA catalysis===
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The human TREX2 3' -&gt; 5'-exonuclease structure suggests a mechanism for efficient nonprocessive DNA catalysis.,Perrino FW, Harvey S, McMillin S, Hollis T J Biol Chem. 2005 Apr 15;280(15):15212-8. Epub 2005 Jan 19. PMID:15661738<ref>PMID:15661738</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1y97" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_15661738}}, adds the Publication Abstract to the page
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*[[Exonuclease 3D structures|Exonuclease 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 15661738 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15661738}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1Y97 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y97 OCA].
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==Reference==
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<ref group="xtra">PMID:15661738</ref><references group="xtra"/>
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[[Category: Exodeoxyribonuclease III]]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Harvey, S.]]
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[[Category: Large Structures]]
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[[Category: Hollis, T.]]
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[[Category: Harvey S]]
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[[Category: McMillin, S.]]
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[[Category: Hollis T]]
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[[Category: Perrino, F W.]]
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[[Category: McMillin S]]
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[[Category: Exonuclease]]
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[[Category: Perrino FW]]
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[[Category: Trex2]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 23:48:30 2009''
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Current revision

The human TREX2 3' exonuclease structure suggests a mechanism for efficient non-processive DNA catalysis

PDB ID 1y97

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