2zqe

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{{Seed}}
 
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[[Image:2zqe.png|left|200px]]
 
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==Crystal structure of the Smr domain of Thermus thermophilus MutS2==
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The line below this paragraph, containing "STRUCTURE_2zqe", creates the "Structure Box" on the page.
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<StructureSection load='2zqe' size='340' side='right'caption='[[2zqe]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2zqe]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZQE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZQE FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zqe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zqe OCA], [https://pdbe.org/2zqe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zqe RCSB], [https://www.ebi.ac.uk/pdbsum/2zqe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zqe ProSAT]</span></td></tr>
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{{STRUCTURE_2zqe| PDB=2zqe | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MUTS2_THET8 MUTS2_THET8] Endonuclease that is involved in the suppression of homologous recombination and may therefore have a key role in the control of bacterial genetic diversity. Cleaves the phosphate backbone of oligodeoxynucleotides non-sequence-specifically at the 3' side of the phosphates. Preferably incises the branched DNA structures, especially the D-loop structure over the Holliday junction. Has ATPase activity. Binds to dsDNA but not to ssDNA.[HAMAP-Rule:MF_00092]<ref>PMID:15113836</ref> <ref>PMID:17215294</ref> <ref>PMID:17686785</ref> <ref>PMID:18838375</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zq/2zqe_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zqe ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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DNA recombination events need to be strictly regulated, because an increase in the recombinational frequency causes unfavorable alteration of genetic information. Recent studies revealed the existence of a novel anti-recombination enzyme, MutS2. However, the mechanism by which MutS2 inhibits homologous recombination has been unknown. Previously, we found that Thermus thermophilus MutS2 (ttMutS2) harbors an endonuclease activity and that this activity is confined to the C-terminal domain, whose amino acid sequence is widely conserved in a variety of proteins with unknown function from almost all organisms ranging from bacteria to man. In this study, we determined the crystal structure of the ttMutS2 endonuclease domain at 1.7-angstroms resolution, which resembles the structure of the DNase I-like catalytic domain of Escherichia coli RNase E, a sequence-nonspecific endonuclease. The N-terminal domain of ttMutS2, however, recognized branched DNA structures, including the Holliday junction and D-loop structure, a primary intermediate in homologous recombination. The full-length of ttMutS2 digested the branched DNA structures at the junction. These results indicate that ttMutS2 suppresses homologous recombination through a novel mechanism involving resolution of early intermediates.
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===Crystal structure of the Smr domain of Thermus thermophilus MutS2===
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Crystal structure of MutS2 endonuclease domain and the mechanism of homologous recombination suppression.,Fukui K, Nakagawa N, Kitamura Y, Nishida Y, Masui R, Kuramitsu S J Biol Chem. 2008 Nov 28;283(48):33417-27. Epub 2008 Oct 6. PMID:18838375<ref>PMID:18838375</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_18838375}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2zqe" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 18838375 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18838375}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2ZQE is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus_hb8 Thermus thermophilus hb8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZQE OCA].
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[[Category: Thermus thermophilus HB8]]
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[[Category: Fukui K]]
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==Reference==
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[[Category: Kitamura Y]]
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<ref group="xtra">PMID:18838375</ref><references group="xtra"/>
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[[Category: Kuramitsu S]]
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[[Category: Thermus thermophilus hb8]]
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[[Category: Masui R]]
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[[Category: Fukui, K.]]
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[[Category: Nakagawa N]]
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[[Category: Kitamura, Y.]]
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[[Category: Kuramitsu, S.]]
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[[Category: Masui, R.]]
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[[Category: Nakagawa, N.]]
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[[Category: Alpha/beta]]
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[[Category: Atp-binding]]
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[[Category: Dna binding protein]]
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[[Category: Dna-binding]]
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[[Category: Nucleotide-binding]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 23:55:25 2009''
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Current revision

Crystal structure of the Smr domain of Thermus thermophilus MutS2

PDB ID 2zqe

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