1zzr

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(New page: 200px<br /><applet load="1zzr" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zzr, resolution 2.05&Aring;" /> '''Rat nNOS D597N/M336V...)
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[[Image:1zzr.gif|left|200px]]<br /><applet load="1zzr" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1zzr, resolution 2.05&Aring;" />
 
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'''Rat nNOS D597N/M336V double mutant with L-N(omega)-Nitroarginine-(4R)-amino-L-proline amide bound'''<br />
 
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==Overview==
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==Rat nNOS D597N/M336V double mutant with L-N(omega)-Nitroarginine-(4R)-amino-L-proline amide bound==
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A series of L-nitroarginine-based dipeptide inhibitors are highly, selective for neuronal nitric oxide synthase (nNOS) over the endothelial, isoform (eNOS). Crystal structures of these dipeptides bound to both, isoforms revealed two different conformations, curled in nNOS and extended, in eNOS, corresponding to higher and lower binding affinity to the two, isoforms, respectively. In previous studies we found that the primary, reason for selectivity is that Asp597 in nNOS, which is Asn368 in eNOS, provides greater electrostatic stabilization in the inhibitor complex., While this is the case for smaller dipeptide inhibitors, electrostatic, stabilization may no longer be the sole determinant for isoform, selectivity with bulkier dipeptide inhibitors. Another residue farther, away from the active site, Met336 in nNOS (Val106 in eNOS), is in contact, with bulkier dipeptide inhibitors. Double mutants were made to exchange, the D597/M336 pair in nNOS with N368/V106 in eNOS. Here we report crystal, structures and inhibition constants for bulkier dipeptide inhibitors bound, to nNOS and eNOS that illustrate the important role played by residues, near the entry to the active site in isoform selective inhibition.
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<StructureSection load='1zzr' size='340' side='right'caption='[[1zzr]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1zzr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZZR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZZR FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DP9:L-N(OMEGA)-NITROARGININE-(4R)-AMINO-L-PROLINE+AMIDE'>DP9</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=H4B:5,6,7,8-TETRAHYDROBIOPTERIN'>H4B</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=TFA:TRIFLUOROACETIC+ACID'>TFA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zzr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zzr OCA], [https://pdbe.org/1zzr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zzr RCSB], [https://www.ebi.ac.uk/pdbsum/1zzr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zzr ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NOS1_RAT NOS1_RAT] Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Inhibitory transmitter for non-adrenergic and non-cholinergic nerves in the colorectum. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. Inhibitory transmitter for non-adrenergic and non-cholinergic nerves in the colorectum.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zz/1zzr_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zzr ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1ZZR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with ZN, HEM, H4B, DP9, TFA and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Nitric-oxide_synthase Nitric-oxide synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.39 1.14.13.39] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ZZR OCA].
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*[[Nitric Oxide Synthase 3D structures|Nitric Oxide Synthase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Exploring the binding conformations of bulkier dipeptide amide inhibitors in constitutive nitric oxide synthases., Li H, Flinspach ML, Igarashi J, Jamal J, Yang W, Gomez-Vidal JA, Litzinger EA, Huang H, Erdal EP, Silverman RB, Poulos TL, Biochemistry. 2005 Nov 22;44(46):15222-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16285725 16285725]
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[[Category: Large Structures]]
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[[Category: Nitric-oxide synthase]]
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[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: Single protein]]
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[[Category: Flinspach ML]]
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[[Category: Flinspach, M.L.]]
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[[Category: Gomez-Vidal JA]]
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[[Category: Gomez-Vidal, J.A.]]
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[[Category: Igarashi J]]
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[[Category: Igarashi, J.]]
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[[Category: Jamal J]]
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[[Category: Jamal, J.]]
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[[Category: Li H]]
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[[Category: Li, H.]]
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[[Category: Litzinger EA]]
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[[Category: Litzinger, E.A.]]
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[[Category: Poulos TL]]
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[[Category: Poulos, T.L.]]
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[[Category: Silverman RB]]
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[[Category: Silverman, R.B.]]
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[[Category: Yang W]]
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[[Category: Yang, W.]]
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[[Category: DP9]]
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[[Category: GOL]]
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[[Category: H4B]]
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[[Category: HEM]]
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[[Category: TFA]]
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[[Category: ZN]]
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[[Category: oxydoreductase enzyme-inhibtor complex]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:45:42 2007''
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Current revision

Rat nNOS D597N/M336V double mutant with L-N(omega)-Nitroarginine-(4R)-amino-L-proline amide bound

PDB ID 1zzr

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