1li7

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{{Seed}}
 
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[[Image:1li7.png|left|200px]]
 
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==Crystal Structure of Cysteinyl-tRNA Synthetase with Cysteine Substrate Bound==
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The line below this paragraph, containing "STRUCTURE_1li7", creates the "Structure Box" on the page.
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<StructureSection load='1li7' size='340' side='right'caption='[[1li7]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1li7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LI7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LI7 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CYS:CYSTEINE'>CYS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_1li7| PDB=1li7 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1li7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1li7 OCA], [https://pdbe.org/1li7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1li7 RCSB], [https://www.ebi.ac.uk/pdbsum/1li7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1li7 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SYC_ECOLI SYC_ECOLI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/li/1li7_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1li7 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Cysteinyl-tRNA synthetase (CysRS) is highly specific for synthesis of cysteinyl adenylate, yet does not possess the amino acid editing activity characteristic of many other tRNA synthetases. To elucidate how CysRS is able to distinguish cysteine from non-cognate amino acids, crystal structures of the Escherichia coli enzyme were determined in apo and cysteine-bound states. The structures reveal that the substrate cysteine thiolate forms a single direct interaction with a zinc ion bound at the base of the active site cleft, in a trigonal bipyramidal geometry together with four highly conserved protein side chains. Cysteine binding induces movement of the zinc ion towards substrate, as well as flipping of the conserved Trp205 indole ring to pack on the thiol side chain. The imidazole groups of five conserved histidines lie adjacent to the zinc ion, forming a unique arrangement suggestive of functional significance. Thus, amino acid discrimination without editing arises most directly from the favorable zinc-thiolate interaction, which is not possible for non-cognate substrates. Additional selectivity may be generated during the induced-fit conformational changes that help assemble the active site.
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===Crystal Structure of Cysteinyl-tRNA Synthetase with Cysteine Substrate Bound===
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Structural origins of amino acid selection without editing by cysteinyl-tRNA synthetase.,Newberry KJ, Hou YM, Perona JJ EMBO J. 2002 Jun 3;21(11):2778-87. PMID:12032090<ref>PMID:12032090</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1li7" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_12032090}}, adds the Publication Abstract to the page
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*[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 12032090 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_12032090}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1LI7 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LI7 OCA].
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==Reference==
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<ref group="xtra">PMID:12032090</ref><references group="xtra"/>
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[[Category: Cysteine--tRNA ligase]]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Hou, Y M.]]
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[[Category: Large Structures]]
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[[Category: Newberry, K J.]]
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[[Category: Hou Y-M]]
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[[Category: Perona, J J.]]
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[[Category: Newberry KJ]]
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[[Category: Cysteine]]
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[[Category: Perona JJ]]
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[[Category: E coli]]
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[[Category: Trna synthetase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 00:26:23 2009''
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Current revision

Crystal Structure of Cysteinyl-tRNA Synthetase with Cysteine Substrate Bound

PDB ID 1li7

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