1b1u

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{{Seed}}
 
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[[Image:1b1u.png|left|200px]]
 
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==CRYSTAL STRUCTURE OF THE BIFUNCTIONAL INHIBITOR RAGI==
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The line below this paragraph, containing "STRUCTURE_1b1u", creates the "Structure Box" on the page.
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<StructureSection load='1b1u' size='340' side='right'caption='[[1b1u]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1b1u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Eleusine_coracana Eleusine coracana]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1jfo 1jfo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B1U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1B1U FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1b1u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b1u OCA], [https://pdbe.org/1b1u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1b1u RCSB], [https://www.ebi.ac.uk/pdbsum/1b1u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1b1u ProSAT]</span></td></tr>
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{{STRUCTURE_1b1u| PDB=1b1u | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IAAT_ELECO IAAT_ELECO] May play a protective role against endo- and exogenous hydrolytic activities in the Ragi seeds.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b1/1b1u_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1b1u ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of a bifunctional inhibitor of alpha-amylase and trypsin (RATI) from ragi seeds (Indian finger millet, Eleusine coracana Gaertneri) has been determined by X-ray diffraction at 2.2 A resolution. The inhibitor consists of 122 amino acids, with five disulfide bridges, and belongs to the plant alpha-amylase/trypsin inhibitor family. The crystals were grown by the microdialysis method using ammonium sulfate as a precipitating agent. The structure was determined by the molecular-replacement method using as models the structures of Corn Hageman factor inhibitor (CHFI) and of RATI at 2.9 A resolution determined previously. It has been refined to an R factor of 21.9%. The structure shows an r.m.s. deviation for C(alpha) atoms of 2.0 A compared with its own NMR structure, whereas the corresponding value compared with CHFI is found to be 1.4 A. The r.m.s. difference for C(alpha) atoms when compared with the same protein in the structure of the complex with alpha-amylase is 0.7 A. The conformations of trypsin-binding loop and the alpha-amylase-binding N-terminal region were also found to be similar in the crystal structures of native RATI and its complex with alpha-amylase. These regions differed considerably in the NMR structure.
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===CRYSTAL STRUCTURE OF THE BIFUNCTIONAL INHIBITOR RAGI===
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Structure of the bifunctional inhibitor of trypsin and alpha-amylase from ragi seeds at 2.2 A resolution.,Gourinath S, Alam N, Srinivasan A, Betzel C, Singh TP Acta Crystallogr D Biol Crystallogr. 2000 Mar;56(Pt 3):287-93. PMID:10713515<ref>PMID:10713515</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_10713515}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1b1u" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 10713515 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_10713515}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1B1U is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Eleusine_coracana Eleusine coracana]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1jfo 1jfo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B1U OCA].
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==Reference==
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<ref group="xtra">PMID:10713515</ref><references group="xtra"/>
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[[Category: Eleusine coracana]]
[[Category: Eleusine coracana]]
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[[Category: Gourinath, S.]]
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[[Category: Large Structures]]
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[[Category: Singh, T P.]]
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[[Category: Gourinath S]]
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[[Category: Srinivasan, A.]]
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[[Category: Singh TP]]
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[[Category: Bifunctional]]
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[[Category: Srinivasan A]]
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[[Category: Hydrolase inhibitor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 00:48:17 2009''
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Current revision

CRYSTAL STRUCTURE OF THE BIFUNCTIONAL INHIBITOR RAGI

PDB ID 1b1u

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