2a2j

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(New page: 200px<br /><applet load="2a2j" size="450" color="white" frame="true" align="right" spinBox="true" caption="2a2j, resolution 2.50&Aring;" /> '''Crystal structure of...)
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[[Image:2a2j.gif|left|200px]]<br /><applet load="2a2j" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2a2j, resolution 2.50&Aring;" />
 
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'''Crystal structure of a putative pyridoxine 5'-phosphate oxidase (Rv2607) from Mycobacterium tuberculosis'''<br />
 
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==Overview==
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==Crystal structure of a putative pyridoxine 5'-phosphate oxidase (Rv2607) from Mycobacterium tuberculosis==
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The three-dimensional structure of Rv2607, a putative pyridoxine, 5'-phosphate oxidase (PNPOx) from Mycobacterium tuberculosis, has been, determined by X-ray crystallography to 2.5 A resolution. Rv2607 has a core, domain similar to known PNPOx structures with a flavin mononucleotide, (FMN) cofactor. Electron density for two FMN at the dimer interface is, weak despite the bright yellow color of the protein solution and crystal., The shape and size of the putative binding pocket is markedly different, from that of members of the PNPOx family, which may indicate some, significant changes in the FMN binding mode of this protein relative to, members of the family.
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<StructureSection load='2a2j' size='340' side='right'caption='[[2a2j]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2a2j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A2J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A2J FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CME:S,S-(2-HYDROXYETHYL)THIOCYSTEINE'>CME</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a2j OCA], [https://pdbe.org/2a2j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a2j RCSB], [https://www.ebi.ac.uk/pdbsum/2a2j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a2j ProSAT], [https://www.topsan.org/Proteins/TBSGC/2a2j TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PDXH_MYCTU PDXH_MYCTU] Catalyzes the oxidation of either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP) (By similarity).[HAMAP-Rule:MF_01629]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a2/2a2j_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a2j ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The three-dimensional structure of Rv2607, a putative pyridoxine 5'-phosphate oxidase (PNPOx) from Mycobacterium tuberculosis, has been determined by X-ray crystallography to 2.5 A resolution. Rv2607 has a core domain similar to known PNPOx structures with a flavin mononucleotide (FMN) cofactor. Electron density for two FMN at the dimer interface is weak despite the bright yellow color of the protein solution and crystal. The shape and size of the putative binding pocket is markedly different from that of members of the PNPOx family, which may indicate some significant changes in the FMN binding mode of this protein relative to members of the family.
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==About this Structure==
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Crystal structure of a putative pyridoxine 5'-phosphate oxidase (Rv2607) from Mycobacterium tuberculosis.,Pedelacq JD, Rho BS, Kim CY, Waldo GS, Lekin TP, Segelke BW, Rupp B, Hung LW, Kim SI, Terwilliger TC Proteins. 2006 Mar 15;62(3):563-9. PMID:16374842<ref>PMID:16374842</ref>
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2A2J is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Active as [http://en.wikipedia.org/wiki/Pyridoxal_5'-phosphate_synthase Pyridoxal 5'-phosphate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.3.5 1.4.3.5] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2A2J OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structure of a putative pyridoxine 5'-phosphate oxidase (Rv2607) from Mycobacterium tuberculosis., Pedelacq JD, Rho BS, Kim CY, Waldo GS, Lekin TP, Segelke BW, Rupp B, Hung LW, Kim SI, Terwilliger TC, Proteins. 2006 Mar 15;62(3):563-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16374842 16374842]
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</div>
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<div class="pdbe-citations 2a2j" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
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[[Category: Pyridoxal 5'-phosphate synthase]]
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[[Category: Hung L-W]]
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[[Category: Single protein]]
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[[Category: Kim C-Y]]
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[[Category: Hung, L.W.]]
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[[Category: Kim S-I]]
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[[Category: Kim, C.Y.]]
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[[Category: Lekin TP]]
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[[Category: Kim, S.I.]]
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[[Category: Pedelacq J-D]]
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[[Category: Lekin, T.P.]]
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[[Category: Rho B-S]]
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[[Category: Pedelacq, J.D.]]
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[[Category: Rupp B]]
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[[Category: Rho, B.S.]]
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[[Category: Segelke BW]]
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[[Category: Rupp, B.]]
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[[Category: Terwilliger TC]]
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[[Category: Segelke, B.W.]]
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[[Category: Waldo GS]]
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[[Category: Terwilliger, T.C.]]
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[[Category: Waldo, G.S.]]
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[[Category: XMTB, Mycobacterium.Tuberculosis.Structural.Proteomics.Project.]]
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[[Category: beta barrel]]
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[[Category: mycobacterium tuberculosis structural proteomics project]]
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[[Category: structural genomics]]
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[[Category: xmtb]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:53:02 2007''
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Current revision

Crystal structure of a putative pyridoxine 5'-phosphate oxidase (Rv2607) from Mycobacterium tuberculosis

PDB ID 2a2j

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