2a7t

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{{Seed}}
 
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[[Image:2a7t.png|left|200px]]
 
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==Crystal Structure of a novel neurotoxin from Buthus tamalus at 2.2A resolution.==
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The line below this paragraph, containing "STRUCTURE_2a7t", creates the "Structure Box" on the page.
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<StructureSection load='2a7t' size='340' side='right'caption='[[2a7t]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2a7t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mesobuthus_tamulus Mesobuthus tamulus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A7T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A7T FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a7t OCA], [https://pdbe.org/2a7t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a7t RCSB], [https://www.ebi.ac.uk/pdbsum/2a7t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a7t ProSAT]</span></td></tr>
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{{STRUCTURE_2a7t| PDB=2a7t | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SCX1_HOTTA SCX1_HOTTA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a7/2a7t_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a7t ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of a highly acidic neurotoxin from the scorpion Buthus tamulus has been determined at 2.2A resolution. The amino acid sequence determination shows that the polypeptide chain has 64 amino acid residues. The pI measurement gave a value of 4.3 which is one of the lowest pI values reported so far for a scorpion toxin. As observed in other alpha-toxins, it contains four disulphide bridges, Cys12-Cys63, Cys16-Cys36, Cys22-Cys46, and Cys26-Cys48. The crystal structure reveals the presence of two crystallographically independent molecules in the asymmetric unit. The conformations of two molecules are identical with an r.m.s. value of 0.3A for their C(alpha) tracings. The overall fold of the toxin is very similar to other scorpion alpha-toxins. It is a betaalphabetabeta protein. The beta-sheet involves residues Glu2-Ile6 (strand beta1), Asp32-Trp39 (strand beta3) and Val45-Val55 (strand beta4). The single alpha-helix formed is by residues Asn19-Asp28 (alpha2). The structure shows a trans peptide bond between residues 9 and 10 in the five-membered reverse turn Asp8-Cys12. This suggests that this toxin belongs to classical alpha-toxin subfamily. The surface features of the present toxin are highly characteristic, the first (A-site) has residues, Phe18, Trp38 and Trp39 that protrude outwardly presumably to interact with its receptor. There is another novel face (N-site) of this neurotoxin that contains several negatively charged residues such as, Glu2, Asp3, Asp32, Glu49 and Asp50 which are clustered in a small region of the toxin structure. On yet another face (P-site) in a triangular arrangement, with respect to the above two faces there are several positively charged residues, Arg58, Lys62 and Arg64 that also protrude outwardly for a potentially potent interaction with other molecules. This toxin with three strong features appears to be one of the most toxic molecules reported so far. In this sense, it may be a new subclass of neurotoxins with the largest number of hot spots.
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===Crystal Structure of a novel neurotoxin from Buthus tamalus at 2.2A resolution.===
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Crystal structure of a highly acidic neurotoxin from scorpion Buthus tamulus at 2.2A resolution reveals novel structural features.,Sharma M, Ethayathulla AS, Jabeen T, Singh N, Sarvanan K, Yadav S, Sharma S, Srinivasan A, Singh TP J Struct Biol. 2006 Jul;155(1):52-62. Epub 2006 Jan 9. PMID:16677826<ref>PMID:16677826</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The line below this paragraph, {{ABSTRACT_PUBMED_16677826}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2a7t" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 16677826 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16677826}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2A7T is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Mesobuthus_tamulus Mesobuthus tamulus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A7T OCA].
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==Reference==
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<ref group="xtra">PMID:16677826</ref><references group="xtra"/>
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[[Category: Mesobuthus tamulus]]
[[Category: Mesobuthus tamulus]]
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[[Category: Ethayathulla, A S.]]
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[[Category: Ethayathulla AS]]
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[[Category: Kaur, P.]]
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[[Category: Kaur P]]
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[[Category: Saravanan, K.]]
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[[Category: Saravanan K]]
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[[Category: Sharma, M.]]
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[[Category: Sharma M]]
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[[Category: Sharma, S.]]
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[[Category: Sharma S]]
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[[Category: Singh, T P.]]
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[[Category: Singh TP]]
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[[Category: Srinivasan, A.]]
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[[Category: Srinivasan A]]
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[[Category: Yadav, S.]]
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[[Category: Yadav S]]
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[[Category: Crystal structure]]
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[[Category: Neurotoxin]]
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[[Category: Novel]]
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[[Category: Red scorpion]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 01:51:07 2009''
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Current revision

Crystal Structure of a novel neurotoxin from Buthus tamalus at 2.2A resolution.

PDB ID 2a7t

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