2ckz

From Proteopedia

(Difference between revisions)

Current revision

X-ray structure of RNA polymerase III subcomplex C17-C25.

Structural highlights

2ckz is a 4 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.2Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RPC9_YEAST DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. The RPC25/RPC8-RPC17/RPC9 subcomplex may bind Pol III transcripts emerging from the adjacent exit pore during elongation.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

We obtained an 11 subunit model of RNA polymerase (Pol) III by combining a homology model of the nine subunit core enzyme with a new X-ray structure of the subcomplex C17/25. Compared to Pol II, Pol III shows a conserved active center for RNA synthesis but a structurally different upstream face for specific initiation complex assembly during promoter selection. The Pol III upstream face includes a HRDC domain in subunit C17 that is translated by 35 A and rotated by 150 degrees compared to its Pol II counterpart. The HRDC domain is essential in vivo, folds independently in vitro, and, unlike other HRDC domains, shows no indication of nucleic acid binding. Thus, the HRDC domain is a functional module that could account for the role of C17 in Pol III promoter-specific initiation. During elongation, C17/25 may bind Pol III transcripts emerging from the adjacent exit pore, because the subcomplex binds to tRNA in vitro.

Structural biology of RNA polymerase III: subcomplex C17/25 X-ray structure and 11 subunit enzyme model.,Jasiak AJ, Armache KJ, Martens B, Jansen RP, Cramer P Mol Cell. 2006 Jul 7;23(1):71-81. PMID:16818233^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Jasiak AJ, Armache KJ, Martens B, Jansen RP, Cramer P. Structural biology of RNA polymerase III: subcomplex C17/25 X-ray structure and 11 subunit enzyme model. Mol Cell. 2006 Jul 7;23(1):71-81. PMID:16818233 doi:10.1016/j.molcel.2006.05.013

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ckz"

@@ Line 1: / Line 1: @@
-{{Seed}}
-[[Image:2ckz.png|left|200px]]
-<!--
+==X-ray structure of RNA polymerase III subcomplex C17-C25.==
-The line below this paragraph, containing "STRUCTURE_2ckz", creates the "Structure Box" on the page.
+<StructureSection load='2ckz' size='340' side='right'caption='[[2ckz]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2ckz]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CKZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CKZ FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ckz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ckz OCA], [https://pdbe.org/2ckz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ckz RCSB], [https://www.ebi.ac.uk/pdbsum/2ckz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ckz ProSAT]</span></td></tr>
-{{STRUCTURE_2ckz|  PDB=2ckz  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/RPC9_YEAST RPC9_YEAST] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. The RPC25/RPC8-RPC17/RPC9 subcomplex may bind Pol III transcripts emerging from the adjacent exit pore during elongation.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ck/2ckz_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ckz ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+We obtained an 11 subunit model of RNA polymerase (Pol) III by combining a homology model of the nine subunit core enzyme with a new X-ray structure of the subcomplex C17/25. Compared to Pol II, Pol III shows a conserved active center for RNA synthesis but a structurally different upstream face for specific initiation complex assembly during promoter selection. The Pol III upstream face includes a HRDC domain in subunit C17 that is translated by 35 A and rotated by 150 degrees compared to its Pol II counterpart. The HRDC domain is essential in vivo, folds independently in vitro, and, unlike other HRDC domains, shows no indication of nucleic acid binding. Thus, the HRDC domain is a functional module that could account for the role of C17 in Pol III promoter-specific initiation. During elongation, C17/25 may bind Pol III transcripts emerging from the adjacent exit pore, because the subcomplex binds to tRNA in vitro.
-===X-RAY STRUCTURE OF RNA POLYMERASE III SUBCOMPLEX C17-C25.===
+Structural biology of RNA polymerase III: subcomplex C17/25 X-ray structure and 11 subunit enzyme model.,Jasiak AJ, Armache KJ, Martens B, Jansen RP, Cramer P Mol Cell. 2006 Jul 7;23(1):71-81. PMID:16818233<ref>PMID:16818233</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2ckz" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_16818233}}, adds the Publication Abstract to the page
+*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 16818233 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_16818233}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-CKZ is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CKZ OCA].
-==Reference==
-<ref group="xtra">PMID:16818233</ref><references group="xtra"/>
-[[Category: DNA-directed RNA polymerase]]
 [[Category: Saccharomyces cerevisiae]]
-[[Category: Armache, K J.]]
+[[Category: Armache K-J]]
-[[Category: Cramer, P.]]
+[[Category: Cramer P]]
-[[Category: Jansen, R P.]]
+[[Category: Jansen R-P]]
-[[Category: Jasiak, A J.]]
+[[Category: Jasiak AJ]]
-[[Category: Martens, B.]]
+[[Category: Martens B]]
-[[Category: Class iii gene transcription]]
-[[Category: Dna-directed rna polymerase]]
-[[Category: Eukaryotic nucleic acid polymerase]]
-[[Category: Hypothetical protein]]
-[[Category: Multiprotein complex]]
-[[Category: Nuclear protein]]
-[[Category: Nucleotidyltransferase]]
-[[Category: Transcription]]
-[[Category: Transferase]]
-[[Category: Trna synthesis]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 01:53:10 2009''

2ckz

From Proteopedia

Current revision

X-ray structure of RNA polymerase III subcomplex C17-C25.

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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