2pll

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (11:04, 30 August 2023) (edit) (undo)
 
(9 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:2pll.png|left|200px]]
 
-
<!--
+
==Crystal structure of perdeuterated human arginase I==
-
The line below this paragraph, containing "STRUCTURE_2pll", creates the "Structure Box" on the page.
+
<StructureSection load='2pll' size='340' side='right'caption='[[2pll]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2pll]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PLL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PLL FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ABH:2(S)-AMINO-6-BORONOHEXANOIC+ACID'>ABH</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
-
{{STRUCTURE_2pll| PDB=2pll | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pll FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pll OCA], [https://pdbe.org/2pll PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pll RCSB], [https://www.ebi.ac.uk/pdbsum/2pll PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pll ProSAT]</span></td></tr>
 +
</table>
 +
== Disease ==
 +
[https://www.uniprot.org/uniprot/ARGI1_HUMAN ARGI1_HUMAN] Defects in ARG1 are the cause of argininemia (ARGIN) [MIM:[https://omim.org/entry/207800 207800]; also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia.<ref>PMID:1463019</ref> <ref>PMID:7649538</ref>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/ARGI1_HUMAN ARGI1_HUMAN]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pl/2pll_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pll ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Arginase is a manganese metalloenzyme that catalyzes the hydrolysis of l-arginine to yield l-ornithine and urea. In order to establish a foundation for future neutron diffraction studies that will provide conclusive structural information regarding proton/deuteron positions in enzyme-inhibitor complexes, we have expressed, purified, assayed, and determined the X-ray crystal structure of perdeuterated (i.e., fully deuterated) human arginase I complexed with 2(S)-amino-6-boronohexanoic acid (ABH) at 1.90A resolution. Prior to the neutron diffraction experiment, it is important to establish that perdeuteration does not cause any unanticipated structural or functional changes. Accordingly, we find that perdeuterated human arginase I exhibits catalytic activity essentially identical to that of the unlabeled enzyme. Additionally, the structure of the perdeuterated human arginase I-ABH complex is identical to that of the corresponding complex with the unlabeled enzyme. Therefore, we conclude that crystals of the perdeuterated human arginase I-ABH complex are suitable for neutron crystallographic study.
-
===Crystal structure of perdeuterated human arginase I===
+
Expression, purification, assay, and crystal structure of perdeuterated human arginase I.,Di Costanzo L, Moulin M, Haertlein M, Meilleur F, Christianson DW Arch Biochem Biophys. 2007 Sep 1;465(1):82-9. Epub 2007 May 21. PMID:17562323<ref>PMID:17562323</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 2pll" style="background-color:#fffaf0;"></div>
-
<!--
+
==See Also==
-
The line below this paragraph, {{ABSTRACT_PUBMED_17562323}}, adds the Publication Abstract to the page
+
*[[Arginase 3D structures|Arginase 3D structures]]
-
(as it appears on PubMed at http://www.pubmed.gov), where 17562323 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_17562323}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
-
2PLL is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PLL OCA].
+
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:17562323</ref><references group="xtra"/>
+
-
[[Category: Arginase]]
+
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Christianson, D W.]]
+
[[Category: Large Structures]]
-
[[Category: Costanzo, L Di.]]
+
[[Category: Christianson DW]]
-
[[Category: Haertlein, M.]]
+
[[Category: Di Costanzo L]]
-
[[Category: Meilleur, F.]]
+
[[Category: Haertlein M]]
-
[[Category: Moulin, M.]]
+
[[Category: Meilleur F]]
-
[[Category: Hydrolase]]
+
[[Category: Moulin M]]
-
[[Category: Perdeuterated protein]]
+
-
[[Category: X-ray structure]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 02:24:26 2009''
+

Current revision

Crystal structure of perdeuterated human arginase I

PDB ID 2pll

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2pll"
Personal tools