2a8v

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="2a8v" size="450" color="white" frame="true" align="right" spinBox="true" caption="2a8v, resolution 2.4&Aring;" /> '''RHO TRANSCRIPTION TER...)
Current revision (07:20, 23 August 2023) (edit) (undo)
 
(17 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2a8v.gif|left|200px]]<br /><applet load="2a8v" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="2a8v, resolution 2.4&Aring;" />
 
-
'''RHO TRANSCRIPTION TERMINATION FACTOR/RNA COMPLEX'''<br />
 
-
==Overview==
+
==RHO TRANSCRIPTION TERMINATION FACTOR/RNA COMPLEX==
-
The E. coli Rho protein disengages newly transcribed RNA from its DNA, template, helping terminate certain transcripts. We have determined the, X-ray crystal structure of the RNA-binding domain of Rho complexed to an, RNA ligand. Filters that screen both ligand size and chemical, functionality line the primary nucleic acid-binding site, imparting, sequence specificity to a generic single-stranded nucleic acid-binding, fold and explaining the preference of Rho for cytosine-rich RNA. The, crystal packing reveals two Rho domain protomers bound to a single RNA, with a single base spacer, suggesting that the strong RNA-binding sites of, Rho may arise from pairing of RNA-binding modules. Dimerization of, symmetric subunits on an asymmetric ligand is developed as a model for, allosteric control in the action of the intact Rho hexamer.
+
<StructureSection load='2a8v' size='340' side='right'caption='[[2a8v]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2a8v]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A8V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A8V FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a8v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a8v OCA], [https://pdbe.org/2a8v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a8v RCSB], [https://www.ebi.ac.uk/pdbsum/2a8v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a8v ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/RHO_ECOLI RHO_ECOLI] Facilitates transcription termination by a mechanism that involves rho binding to the nascent RNA, activation of rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template. RNA-dependent NTPAse which utilizes all four ribonucleoside triphosphates as substrates.[HAMAP-Rule:MF_01884]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a8/2a8v_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a8v ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The E. coli Rho protein disengages newly transcribed RNA from its DNA template, helping terminate certain transcripts. We have determined the X-ray crystal structure of the RNA-binding domain of Rho complexed to an RNA ligand. Filters that screen both ligand size and chemical functionality line the primary nucleic acid-binding site, imparting sequence specificity to a generic single-stranded nucleic acid-binding fold and explaining the preference of Rho for cytosine-rich RNA. The crystal packing reveals two Rho domain protomers bound to a single RNA with a single base spacer, suggesting that the strong RNA-binding sites of Rho may arise from pairing of RNA-binding modules. Dimerization of symmetric subunits on an asymmetric ligand is developed as a model for allosteric control in the action of the intact Rho hexamer.
-
==About this Structure==
+
The structural basis for terminator recognition by the Rho transcription termination factor.,Bogden CE, Fass D, Bergman N, Nichols MD, Berger JM Mol Cell. 1999 Apr;3(4):487-93. PMID:10230401<ref>PMID:10230401</ref>
-
2A8V is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2A8V OCA].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
The structural basis for terminator recognition by the Rho transcription termination factor., Bogden CE, Fass D, Bergman N, Nichols MD, Berger JM, Mol Cell. 1999 Apr;3(4):487-93. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10230401 10230401]
+
</div>
-
[[Category: Escherichia coli]]
+
<div class="pdbe-citations 2a8v" style="background-color:#fffaf0;"></div>
-
[[Category: Single protein]]
+
-
[[Category: Berger, J.M.]]
+
-
[[Category: Bergman, N.]]
+
-
[[Category: Bogden, C.E.]]
+
-
[[Category: Fass, D.]]
+
-
[[Category: Nichols, M.D.]]
+
-
[[Category: ob fold]]
+
-
[[Category: protein/rna complex]]
+
-
[[Category: rho]]
+
-
[[Category: single-stranded nucleic acid binding domain]]
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:59:23 2007''
+
==See Also==
 +
*[[Helicase 3D structures|Helicase 3D structures]]
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Escherichia coli]]
 +
[[Category: Escherichia coli K-12]]
 +
[[Category: Large Structures]]
 +
[[Category: Berger JM]]
 +
[[Category: Bergman N]]
 +
[[Category: Bogden CE]]
 +
[[Category: Fass D]]
 +
[[Category: Nichols MD]]

Current revision

RHO TRANSCRIPTION TERMINATION FACTOR/RNA COMPLEX

PDB ID 2a8v

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2a8v"
Personal tools