1s0v

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{{Seed}}
 
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[[Image:1s0v.png|left|200px]]
 
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==Structural basis for substrate selection by T7 RNA polymerase==
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The line below this paragraph, containing "STRUCTURE_1s0v", creates the "Structure Box" on the page.
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<StructureSection load='1s0v' size='340' side='right'caption='[[1s0v]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1s0v]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_phage_T7 Escherichia phage T7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S0V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S0V FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=APC:DIPHOSPHOMETHYLPHOSPHONIC+ACID+ADENOSYL+ESTER'>APC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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{{STRUCTURE_1s0v| PDB=1s0v | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s0v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s0v OCA], [https://pdbe.org/1s0v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s0v RCSB], [https://www.ebi.ac.uk/pdbsum/1s0v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s0v ProSAT], [https://www.topsan.org/Proteins/RSGI/1s0v TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RPOL_BPT7 RPOL_BPT7] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Responsible for the transcription of the late genes of T7. It is rifampicin-resistant. It recognizes a specific promoter sequence, unwinds the double-stranded RNA to expose the coding strand for templating, initiates transcription preferentially with a purine.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/s0/1s0v_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1s0v ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The mechanism by which nucleotide polymerases select the correct substrate is of fundamental importance to the fidelity of DNA replication and transcription. During the nucleotide addition cycle, pol I DNA polymerases undergo the transition from a catalytically inactive "open" to an active "closed" conformation. All known determinants of substrate selection are associated with the "closed" state. To elucidate if this mechanism is conserved in homologous single subunit RNA polymerases (RNAPs), we have determined the structure of T7 RNAP elongation complex with the incoming substrate analog. Surprisingly, the substrate specifically binds to RNAP in the "open" conformation, where it is base paired with the acceptor template base, while Tyr639 provides discrimination of ribose versus deoxyribose substrates. The structure therefore suggests a novel mechanism, in which the substrate selection occurs prior to the isomerization to the catalytically active conformation. Modeling of multisubunit RNAPs suggests that this mechanism might be universal for all RNAPs.
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===Structural basis for substrate selection by T7 RNA polymerase===
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Structural basis for substrate selection by t7 RNA polymerase.,Temiakov D, Patlan V, Anikin M, McAllister WT, Yokoyama S, Vassylyev DG Cell. 2004 Feb 6;116(3):381-91. PMID:15016373<ref>PMID:15016373</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1s0v" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_15016373}}, adds the Publication Abstract to the page
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*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 15016373 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15016373}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Escherichia phage T7]]
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1S0V is a 16 chains structure of sequences from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S0V OCA].
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[[Category: Large Structures]]
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[[Category: Anikin M]]
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==Reference==
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[[Category: McAllister WT]]
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<ref group="xtra">PMID:15016373</ref><references group="xtra"/>
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[[Category: Patlan V]]
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[[Category: DNA-directed RNA polymerase]]
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[[Category: Temiakov D]]
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[[Category: Enterobacteria phage t7]]
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[[Category: Vassylyev DG]]
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[[Category: Anikin, M.]]
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[[Category: Yokoyama S]]
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[[Category: McAllister, W T.]]
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[[Category: Patlan, V.]]
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[[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]]
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[[Category: Temiakov, D.]]
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[[Category: Vassylyev, D G.]]
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[[Category: Yokoyama, S.]]
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[[Category: Dna]]
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[[Category: Riken structural genomics/proteomics initiative]]
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[[Category: Rna]]
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[[Category: Rsgi]]
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[[Category: Structural genomic]]
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[[Category: T7 rna polymerase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 02:56:19 2009''
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Current revision

Structural basis for substrate selection by T7 RNA polymerase

PDB ID 1s0v

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