2aa9

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(New page: 200px<br /><applet load="2aa9" size="450" color="white" frame="true" align="right" spinBox="true" caption="2aa9, resolution 1.5&Aring;" /> '''EPSP synthase ligande...)
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[[Image:2aa9.gif|left|200px]]<br /><applet load="2aa9" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2aa9, resolution 1.5&Aring;" />
 
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'''EPSP synthase liganded with shikimate'''<br />
 
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==Overview==
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==EPSP synthase liganded with shikimate==
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The shikimate pathway enzyme 5-enolpyruvyl shikimate-3-phosphate synthase, (EPSP synthase) has received attention in the past because it is the, target of the broad-spectrum herbicide glyphosate. The natural substrate, of EPSP synthase is shikimate-3-phosphate. However, this enzyme can also, utilize shikimate as substrate. Remarkably, this reaction is insensitive, to inhibition by glyphosate. Crystallographic analysis of EPSP synthase, from Escherichia coli, in complex with shikimate/glyphosate at 1.5, Angstroms resolution, revealed that binding of shikimate induces changes, around the backbone of the active site, which in turn impact the efficient, binding of glyphosate. The implications from these findings with respect, to the design of novel glyphosate-insensitive EPSP synthase enzymes are, discussed.
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<StructureSection load='2aa9' size='340' side='right'caption='[[2aa9]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2aa9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AA9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AA9 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=SKM:(3R,4S,5R)-3,4,5-TRIHYDROXYCYCLOHEX-1-ENE-1-CARBOXYLIC+ACID'>SKM</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2aa9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aa9 OCA], [https://pdbe.org/2aa9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2aa9 RCSB], [https://www.ebi.ac.uk/pdbsum/2aa9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2aa9 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AROA_ECOLI AROA_ECOLI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/aa/2aa9_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2aa9 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The shikimate pathway enzyme 5-enolpyruvyl shikimate-3-phosphate synthase (EPSP synthase) has received attention in the past because it is the target of the broad-spectrum herbicide glyphosate. The natural substrate of EPSP synthase is shikimate-3-phosphate. However, this enzyme can also utilize shikimate as substrate. Remarkably, this reaction is insensitive to inhibition by glyphosate. Crystallographic analysis of EPSP synthase from Escherichia coli, in complex with shikimate/glyphosate at 1.5 Angstroms resolution, revealed that binding of shikimate induces changes around the backbone of the active site, which in turn impact the efficient binding of glyphosate. The implications from these findings with respect to the design of novel glyphosate-insensitive EPSP synthase enzymes are discussed.
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==About this Structure==
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Molecular basis for the glyphosate-insensitivity of the reaction of 5-enolpyruvylshikimate 3-phosphate synthase with shikimate.,Priestman MA, Healy ML, Funke T, Becker A, Schonbrunn E FEBS Lett. 2005 Oct 24;579(25):5773-80. PMID:16225867<ref>PMID:16225867</ref>
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2AA9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with SKM and FMT as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/3-phosphoshikimate_1-carboxyvinyltransferase 3-phosphoshikimate 1-carboxyvinyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.19 2.5.1.19] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2AA9 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Molecular basis for the glyphosate-insensitivity of the reaction of 5-enolpyruvylshikimate 3-phosphate synthase with shikimate., Priestman MA, Healy ML, Funke T, Becker A, Schonbrunn E, FEBS Lett. 2005 Oct 24;579(25):5773-80. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16225867 16225867]
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</div>
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[[Category: 3-phosphoshikimate 1-carboxyvinyltransferase]]
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<div class="pdbe-citations 2aa9" style="background-color:#fffaf0;"></div>
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[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Becker, A.]]
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[[Category: Funke, T.]]
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[[Category: Healy, M.L.]]
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[[Category: Priestman, M.A.]]
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[[Category: Schonbrunn, E.]]
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[[Category: FMT]]
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[[Category: SKM]]
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[[Category: inside-out alpha/beta barrel]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 08:00:41 2007''
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==See Also==
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*[[EPSP synthase 3D structures|EPSP synthase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Escherichia coli]]
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[[Category: Large Structures]]
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[[Category: Becker A]]
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[[Category: Funke T]]
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[[Category: Healy ML]]
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[[Category: Priestman MA]]
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[[Category: Schonbrunn E]]

Current revision

EPSP synthase liganded with shikimate

PDB ID 2aa9

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