2aay

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(New page: 200px<br /><applet load="2aay" size="450" color="white" frame="true" align="right" spinBox="true" caption="2aay, resolution 1.55&Aring;" /> '''EPSP synthase ligand...)
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[[Image:2aay.gif|left|200px]]<br /><applet load="2aay" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2aay, resolution 1.55&Aring;" />
 
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'''EPSP synthase liganded with shikimate and glyphosate'''<br />
 
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==Overview==
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==EPSP synthase liganded with shikimate and glyphosate==
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The shikimate pathway enzyme 5-enolpyruvyl shikimate-3-phosphate synthase, (EPSP synthase) has received attention in the past because it is the, target of the broad-spectrum herbicide glyphosate. The natural substrate, of EPSP synthase is shikimate-3-phosphate. However, this enzyme can also, utilize shikimate as substrate. Remarkably, this reaction is insensitive, to inhibition by glyphosate. Crystallographic analysis of EPSP synthase, from Escherichia coli, in complex with shikimate/glyphosate at 1.5, Angstroms resolution, revealed that binding of shikimate induces changes, around the backbone of the active site, which in turn impact the efficient, binding of glyphosate. The implications from these findings with respect, to the design of novel glyphosate-insensitive EPSP synthase enzymes are, discussed.
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<StructureSection load='2aay' size='340' side='right'caption='[[2aay]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2aay]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AAY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AAY FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GPJ:GLYPHOSATE'>GPJ</scene>, <scene name='pdbligand=SKM:(3R,4S,5R)-3,4,5-TRIHYDROXYCYCLOHEX-1-ENE-1-CARBOXYLIC+ACID'>SKM</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2aay FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aay OCA], [https://pdbe.org/2aay PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2aay RCSB], [https://www.ebi.ac.uk/pdbsum/2aay PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2aay ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AROA_ECOLI AROA_ECOLI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/aa/2aay_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2aay ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The shikimate pathway enzyme 5-enolpyruvyl shikimate-3-phosphate synthase (EPSP synthase) has received attention in the past because it is the target of the broad-spectrum herbicide glyphosate. The natural substrate of EPSP synthase is shikimate-3-phosphate. However, this enzyme can also utilize shikimate as substrate. Remarkably, this reaction is insensitive to inhibition by glyphosate. Crystallographic analysis of EPSP synthase from Escherichia coli, in complex with shikimate/glyphosate at 1.5 Angstroms resolution, revealed that binding of shikimate induces changes around the backbone of the active site, which in turn impact the efficient binding of glyphosate. The implications from these findings with respect to the design of novel glyphosate-insensitive EPSP synthase enzymes are discussed.
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==About this Structure==
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Molecular basis for the glyphosate-insensitivity of the reaction of 5-enolpyruvylshikimate 3-phosphate synthase with shikimate.,Priestman MA, Healy ML, Funke T, Becker A, Schonbrunn E FEBS Lett. 2005 Oct 24;579(25):5773-80. PMID:16225867<ref>PMID:16225867</ref>
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2AAY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with SKM, GPJ and FMT as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/3-phosphoshikimate_1-carboxyvinyltransferase 3-phosphoshikimate 1-carboxyvinyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.19 2.5.1.19] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2AAY OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Molecular basis for the glyphosate-insensitivity of the reaction of 5-enolpyruvylshikimate 3-phosphate synthase with shikimate., Priestman MA, Healy ML, Funke T, Becker A, Schonbrunn E, FEBS Lett. 2005 Oct 24;579(25):5773-80. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16225867 16225867]
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</div>
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[[Category: 3-phosphoshikimate 1-carboxyvinyltransferase]]
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<div class="pdbe-citations 2aay" style="background-color:#fffaf0;"></div>
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[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Becker, A.]]
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[[Category: Funke, T.]]
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[[Category: Healy, M.L.]]
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[[Category: Priestman, M.A.]]
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[[Category: FMT]]
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[[Category: GPJ]]
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[[Category: SKM]]
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[[Category: inside-out alpha/beta barrel]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 08:01:40 2007''
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==See Also==
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*[[EPSP synthase 3D structures|EPSP synthase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Escherichia coli]]
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[[Category: Large Structures]]
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[[Category: Becker A]]
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[[Category: Funke T]]
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[[Category: Healy ML]]
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[[Category: Priestman MA]]

Current revision

EPSP synthase liganded with shikimate and glyphosate

PDB ID 2aay

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