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2afj

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(New page: 200px<br /><applet load="2afj" size="450" color="white" frame="true" align="right" spinBox="true" caption="2afj" /> '''SPRY domain-containing SOCS box protein 2 (S...)
Current revision (08:17, 15 May 2024) (edit) (undo)
 
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[[Image:2afj.gif|left|200px]]<br /><applet load="2afj" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2afj" />
 
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'''SPRY domain-containing SOCS box protein 2 (SSB-2)'''<br />
 
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==Overview==
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==SPRY domain-containing SOCS box protein 2 (SSB-2)==
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The four mammalian SPRY domain-containing SOCS box proteins (SSB-1 to, SSB-4) are characterized by a C-terminal SOCS box and a central SPRY, domain. We have determined the first SPRY-domain structure, as part of, SSB-2, by NMR. This domain adopts a novel fold consisting of a, beta-sandwich structure formed by two four-stranded antiparallel, beta-sheets with a unique topology. We demonstrate that SSB-1, SSB-2 and, SSB-4, but not SSB-3, bind prostate apoptosis response protein-4 (Par-4)., Mutational analysis of SSB-2 loop regions identified conserved structural, determinants for its interaction with Par-4 and the hepatocyte growth, factor receptor, c-Met. Mutations in analogous loop regions of pyrin and, midline-1 SPRY domains have been shown to cause Mediterranean fever and, Opitz syndrome, respectively. Our findings provide a template for, SPRY-domain structure and an insight into the mechanism of SPRY-protein, interaction.
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<StructureSection load='2afj' size='340' side='right'caption='[[2afj]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2afj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AFJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AFJ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2afj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2afj OCA], [https://pdbe.org/2afj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2afj RCSB], [https://www.ebi.ac.uk/pdbsum/2afj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2afj ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SPSB2_MOUSE SPSB2_MOUSE] Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/af/2afj_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2afj ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The four mammalian SPRY domain-containing SOCS box proteins (SSB-1 to SSB-4) are characterized by a C-terminal SOCS box and a central SPRY domain. We have determined the first SPRY-domain structure, as part of SSB-2, by NMR. This domain adopts a novel fold consisting of a beta-sandwich structure formed by two four-stranded antiparallel beta-sheets with a unique topology. We demonstrate that SSB-1, SSB-2 and SSB-4, but not SSB-3, bind prostate apoptosis response protein-4 (Par-4). Mutational analysis of SSB-2 loop regions identified conserved structural determinants for its interaction with Par-4 and the hepatocyte growth factor receptor, c-Met. Mutations in analogous loop regions of pyrin and midline-1 SPRY domains have been shown to cause Mediterranean fever and Opitz syndrome, respectively. Our findings provide a template for SPRY-domain structure and an insight into the mechanism of SPRY-protein interaction.
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==About this Structure==
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The SPRY domain of SSB-2 adopts a novel fold that presents conserved Par-4-binding residues.,Masters SL, Yao S, Willson TA, Zhang JG, Palmer KR, Smith BJ, Babon JJ, Nicola NA, Norton RS, Nicholson SE Nat Struct Mol Biol. 2006 Jan;13(1):77-84. Epub 2005 Dec 20. PMID:16369487<ref>PMID:16369487</ref>
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2AFJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2AFJ OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The SPRY domain of SSB-2 adopts a novel fold that presents conserved Par-4-binding residues., Masters SL, Yao S, Willson TA, Zhang JG, Palmer KR, Smith BJ, Babon JJ, Nicola NA, Norton RS, Nicholson SE, Nat Struct Mol Biol. 2006 Jan;13(1):77-84. Epub 2005 Dec 20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16369487 16369487]
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</div>
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<div class="pdbe-citations 2afj" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Single protein]]
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[[Category: Babon JJ]]
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[[Category: Babon, J.J.]]
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[[Category: Masters SL]]
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[[Category: Masters, S.L.]]
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[[Category: Nicholson SE]]
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[[Category: Nicholson, S.E.]]
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[[Category: Nicola NA]]
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[[Category: Nicola, N.A.]]
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[[Category: Norton RS]]
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[[Category: Norton, R.S.]]
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[[Category: Palmer KR]]
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[[Category: Palmer, K.R.]]
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[[Category: Smith BJ]]
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[[Category: Smith, B.J.]]
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[[Category: Willson TA]]
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[[Category: Willson, T.A.]]
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[[Category: Yao S]]
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[[Category: Yao, S.]]
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[[Category: Zhang JG]]
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[[Category: Zhang, J.G.]]
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[[Category: beta sandwich]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 08:05:49 2007''
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Current revision

SPRY domain-containing SOCS box protein 2 (SSB-2)

PDB ID 2afj

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