1jbt

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{{Seed}}
 
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[[Image:1jbt.png|left|200px]]
 
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==CRYSTAL STRUCTURE OF RIBOTOXIN RESTRICTOCIN COMPLEXED WITH A 29-MER SARCIN/RICIN DOMAIN RNA ANALOG==
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The line below this paragraph, containing "STRUCTURE_1jbt", creates the "Structure Box" on the page.
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<StructureSection load='1jbt' size='340' side='right'caption='[[1jbt]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1jbt]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_restrictus Aspergillus restrictus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JBT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JBT FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
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{{STRUCTURE_1jbt| PDB=1jbt | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jbt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jbt OCA], [https://pdbe.org/1jbt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jbt RCSB], [https://www.ebi.ac.uk/pdbsum/1jbt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jbt ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RNMG_ASPRE RNMG_ASPRE] This purine-specific ribonuclease cleaves 28S RNA in eukaryotic ribosomes, inhibits protein synthesis, and shows antitumor activity.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jb/1jbt_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jbt ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The cytotoxin sarcin disrupts elongation factor binding and protein synthesis by specifically cleaving one phosphodiester bond in ribosomes. To elucidate the molecular basis of toxin action, we determined three cocrystal structures of the sarcin homolog restrictocin bound to different analogs that mimic the target sarcin/ricin loop (SRL) structure of the rat 28S rRNA. In these structures, restrictocin contacts the bulged-G motif and an unfolded form of the tetraloop of the SRL RNA. In one structure, toxin loops guide selection of the target site by contacting the base critical for recognition (G4319) and the surrounding S-shaped backbone. In another structure, base flipping of the tetraloop enables cleavage by placing the target nucleotide in the active site with the nucleophile nearly inline for attack on the scissile bond. These structures provide the first views of how a site-specific protein endonuclease recognizes and cleaves a folded RNA substrate.
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===CRYSTAL STRUCTURE OF RIBOTOXIN RESTRICTOCIN COMPLEXED WITH A 29-MER SARCIN/RICIN DOMAIN RNA ANALOG===
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Crystal structures of restrictocin-inhibitor complexes with implications for RNA recognition and base flipping.,Yang X, Gerczei T, Glover LT, Correll CC Nat Struct Biol. 2001 Nov;8(11):968-73. PMID:11685244<ref>PMID:11685244</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_11685244}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1jbt" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 11685244 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_11685244}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1JBT is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Aspergillus_restrictus Aspergillus restrictus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JBT OCA].
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==Reference==
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<ref group="xtra">PMID:11685244</ref><references group="xtra"/>
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[[Category: Aspergillus restrictus]]
[[Category: Aspergillus restrictus]]
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[[Category: Correll, C C.]]
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[[Category: Large Structures]]
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[[Category: Gerczei, T.]]
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[[Category: Correll CC]]
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[[Category: Glover, L.]]
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[[Category: Gerczei T]]
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[[Category: Yang, X.]]
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[[Category: Glover L]]
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[[Category: Highly specific ribonuclease]]
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[[Category: Yang X]]
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[[Category: Protein-rna recognition]]
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[[Category: Restrictocin]]
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[[Category: Ribonuclease t1]]
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[[Category: Ribotoxin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 04:29:45 2009''
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Current revision

CRYSTAL STRUCTURE OF RIBOTOXIN RESTRICTOCIN COMPLEXED WITH A 29-MER SARCIN/RICIN DOMAIN RNA ANALOG

PDB ID 1jbt

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