1f39

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[[Image:1f39.png|left|200px]]
 
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==CRYSTAL STRUCTURE OF THE LAMBDA REPRESSOR C-TERMINAL DOMAIN==
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The line below this paragraph, containing "STRUCTURE_1f39", creates the "Structure Box" on the page.
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<StructureSection load='1f39' size='340' side='right'caption='[[1f39]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1f39]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_Lambda Escherichia virus Lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F39 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F39 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f39 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f39 OCA], [https://pdbe.org/1f39 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f39 RCSB], [https://www.ebi.ac.uk/pdbsum/1f39 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f39 ProSAT]</span></td></tr>
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{{STRUCTURE_1f39| PDB=1f39 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RPC1_LAMBD RPC1_LAMBD] Repressor protein CI allows phage lambda to reside inactively in the chromosome of its host bacterium. This lysogenic state is maintained by binding of regulatory protein CI to the OR and OL operators, preventing transcription of proteins necessary for lytic development.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f3/1f39_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1f39 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Interactions between transcription factors bound to separate operator sites commonly play an important role in gene regulation by mediating cooperative binding to the DNA. However, few detailed structural models for understanding the molecular basis of such cooperativity are available. The c1 repressor of bacteriophage lambda is a classic example of a protein that binds to its operator sites cooperatively. The C-terminal domain of the repressor mediates dimerization as well as a dimer-dimer interaction that results in the cooperative binding of two repressor dimers to adjacent operator sites. Here, we present the x-ray crystal structure of the lambda repressor C-terminal domain determined by multiwavelength anomalous diffraction. Remarkably, the interactions that mediate cooperativity are captured in the crystal, where two dimers associate about a 2-fold axis of symmetry. Based on the structure and previous genetic and biochemical data, we present a model for the cooperative binding of two lambda repressor dimers at adjacent operator sites.
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===CRYSTAL STRUCTURE OF THE LAMBDA REPRESSOR C-TERMINAL DOMAIN===
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Crystal structure of the lambda repressor C-terminal domain provides a model for cooperative operator binding.,Bell CE, Frescura P, Hochschild A, Lewis M Cell. 2000 Jun 23;101(7):801-11. PMID:10892750<ref>PMID:10892750</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_10892750}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1f39" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 10892750 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_10892750}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Escherichia virus Lambda]]
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1F39 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F39 OCA].
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[[Category: Large Structures]]
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[[Category: Bell CE]]
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==Reference==
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[[Category: Frescura P]]
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<ref group="xtra">PMID:10892750</ref><references group="xtra"/>
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[[Category: Hochschild A]]
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[[Category: Enterobacteria phage lambda]]
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[[Category: Lewis M]]
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[[Category: Bell, C E.]]
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[[Category: Frescura, P.]]
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[[Category: Hochschild, A.]]
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[[Category: Lewis, M.]]
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[[Category: Cooperative operator binding]]
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[[Category: Gene regulation]]
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[[Category: Reca-mediated self-cleavage]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 04:32:42 2009''
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Current revision

CRYSTAL STRUCTURE OF THE LAMBDA REPRESSOR C-TERMINAL DOMAIN

PDB ID 1f39

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