2ak3

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(New page: 200px<br /><applet load="2ak3" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ak3, resolution 1.85&Aring;" /> '''THE THREE-DIMENSIONA...)
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[[Image:2ak3.gif|left|200px]]<br /><applet load="2ak3" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2ak3, resolution 1.85&Aring;" />
 
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'''THE THREE-DIMENSIONAL STRUCTURE OF THE COMPLEX BETWEEN MITOCHONDRIAL MATRIX ADENYLATE KINASE AND ITS SUBSTRATE AMP AT 1.85 ANGSTROMS RESOLUTION'''<br />
 
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==Overview==
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==THE THREE-DIMENSIONAL STRUCTURE OF THE COMPLEX BETWEEN MITOCHONDRIAL MATRIX ADENYLATE KINASE AND ITS SUBSTRATE AMP AT 1.85 ANGSTROMS RESOLUTION==
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The crystal structure of the complex between adenylate kinase from bovine, mitochondrial matrix and its substrate AMP has been refined at 1.85 A, resolution (1 A = 0.1 nm). Based on 42,519 independent reflections of, better than 10 A resolution, a final R-factor of 18.9% was obtained with a, model obeying standard geometry within 0.016 A in bond lengths and 3.2, degrees in bond angles. There are two enzyme: substrate complexes in the, asymmetric unit, each consisting of 226 amino acid residues, one AMP and, one sulfate ion. A superposition of the two full-length polypeptides, revealed deviations that can be described as small relative movements of, three domains. Best superpositions of individual domains yielded a, residual overall root-mean-square deviation of 0.3 A for the backbone, atoms and 0.5 A for the sidechains. The final model contains 381 solvent, molecules in the asymmetric unit, 2 x 72 = 144 of which occupy, corresponding positions in both complexes.
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<StructureSection load='2ak3' size='340' side='right'caption='[[2ak3]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2ak3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1ak3 1ak3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AK3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AK3 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ak3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ak3 OCA], [https://pdbe.org/2ak3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ak3 RCSB], [https://www.ebi.ac.uk/pdbsum/2ak3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ak3 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/KAD3_BOVIN KAD3_BOVIN]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ak/2ak3_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ak3 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2AK3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with SO4 and AMP as [http://en.wikipedia.org/wiki/ligands ligands]. This structure superseeds the now removed PDB entry 1AK3. Active as [http://en.wikipedia.org/wiki/Nucleoside-triphosphate--adenylate_kinase Nucleoside-triphosphate--adenylate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.10 2.7.4.10] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2AK3 OCA].
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*[[Adenylate kinase 3D structures|Adenylate kinase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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The refined structure of the complex between adenylate kinase from beef heart mitochondrial matrix and its substrate AMP at 1.85 A resolution., Diederichs K, Schulz GE, J Mol Biol. 1991 Feb 5;217(3):541-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1994037 1994037]
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[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Nucleoside-triphosphate--adenylate kinase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Diederichs K]]
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[[Category: Diederichs, K.]]
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[[Category: Schulz GE]]
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[[Category: Schulz, G.E.]]
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[[Category: AMP]]
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[[Category: SO4]]
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[[Category: transferase (phosphotransferase)]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 08:10:52 2007''
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Current revision

THE THREE-DIMENSIONAL STRUCTURE OF THE COMPLEX BETWEEN MITOCHONDRIAL MATRIX ADENYLATE KINASE AND ITS SUBSTRATE AMP AT 1.85 ANGSTROMS RESOLUTION

PDB ID 2ak3

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