This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2akh

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="2akh" size="450" color="white" frame="true" align="right" spinBox="true" caption="2akh" /> '''Normal mode-based flexible fitted coordinate...)
Current revision (09:13, 14 February 2024) (edit) (undo)
 
(15 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2akh.gif|left|200px]]<br /><applet load="2akh" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="2akh" />
 
-
'''Normal mode-based flexible fitted coordinates of a non-translocating SecYEG protein-conducting channel into the cryo-EM map of a SecYEG-nascent chain-70S ribosome complex from E. coli'''<br />
 
-
==Overview==
+
==Normal mode-based flexible fitted coordinates of a non-translocating SecYEG protein-conducting channel into the cryo-EM map of a SecYEG-nascent chain-70S ribosome complex from E. coli==
-
Secreted and membrane proteins are translocated across or into cell, membranes through a protein-conducting channel (PCC). Here we present a, cryo-electron microscopy reconstruction of the Escherichia coli PCC, SecYEG, complexed with the ribosome and a nascent chain containing a, signal anchor. This reconstruction shows a messenger RNA, three transfer, RNAs, the nascent chain, and detailed features of both a translocating PCC, and a second, non-translocating PCC bound to mRNA hairpins. The, translocating PCC forms connections with ribosomal RNA hairpins on two, sides and ribosomal proteins at the back, leaving a frontal opening., Normal mode-based flexible fitting of the archaeal SecYEbeta structure, into the PCC electron microscopy densities favours a front-to-front, arrangement of two SecYEG complexes in the PCC, and supports channel, formation by the opening of two linked SecY halves during polypeptide, translocation. On the basis of our observation in the translocating PCC of, two segregated pores with different degrees of access to bulk lipid, we, propose a model for co-translational protein translocation.
+
<SX load='2akh' size='340' side='right' viewer='molstar' caption='[[2akh]], [[Resolution|resolution]] 14.90&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2akh]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AKH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AKH FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 14.9&#8491;</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2akh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2akh OCA], [https://pdbe.org/2akh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2akh RCSB], [https://www.ebi.ac.uk/pdbsum/2akh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2akh ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/SECG_ECOLI SECG_ECOLI] Subunit of the protein translocation channel SecYEG. Overexpression of some hybrid proteins has been thought to jam the protein secretion apparatus resulting in cell death; while this may be true it also results in FtsH-mediated degradation of SecY. Treatment with antibiotics that block translation elongation such as chloramphenicol also leads to degradation of SecY and SecE but not SecG.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ak/2akh_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2akh ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
2AKH is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2AKH OCA].
+
*[[Preprotein translocase 3D structures|Preprotein translocase 3D structures]]
-
 
+
__TOC__
-
==Reference==
+
</SX>
-
Structure of the E. coli protein-conducting channel bound to a translating ribosome., Mitra K, Schaffitzel C, Shaikh T, Tama F, Jenni S, Brooks CL 3rd, Ban N, Frank J, Nature. 2005 Nov 17;438(7066):318-24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16292303 16292303]
+
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
-
[[Category: Protein complex]]
+
[[Category: Large Structures]]
-
[[Category: Ban, N.]]
+
[[Category: Ban N]]
-
[[Category: Frank, J.]]
+
[[Category: Brooks III CL]]
-
[[Category: III, C.L.Brooks.]]
+
[[Category: Frank J]]
-
[[Category: Jenni, S.]]
+
[[Category: Jenni S]]
-
[[Category: Mitra, K.M.]]
+
[[Category: Mitra KM]]
-
[[Category: Schaffitzel, C.]]
+
[[Category: Schaffitzel C]]
-
[[Category: Shaikh, T.]]
+
[[Category: Shaikh T]]
-
[[Category: Tama, F.]]
+
[[Category: Tama F]]
-
[[Category: protein transport]]
+
-
[[Category: translocation]]
+
-
[[Category: transmembrane]]
+
-
[[Category: transport]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 08:11:10 2007''
+

Current revision

Normal mode-based flexible fitted coordinates of a non-translocating SecYEG protein-conducting channel into the cryo-EM map of a SecYEG-nascent chain-70S ribosome complex from E. coli

2akh, resolution 14.90Å

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2akh"
Personal tools