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2ane

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(New page: 200px<br /><applet load="2ane" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ane, resolution 2.03&Aring;" /> '''Crystal structure of...)
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[[Image:2ane.gif|left|200px]]<br /><applet load="2ane" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2ane, resolution 2.03&Aring;" />
 
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'''Crystal structure of N-terminal domain of E.Coli Lon Protease'''<br />
 
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==Overview==
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==Crystal structure of N-terminal domain of E.Coli Lon Protease==
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We report here the first crystal structure of the N-terminal domain of an, A-type Lon protease. Lon proteases are ubiquitous, multidomain, ATP-dependent enzymes with both highly specific and non-specific protein, binding, unfolding, and degrading activities. We expressed and purified a, stable, monomeric 119-amino acid N-terminal subdomain of the Escherichia, coli A-type Lon protease and determined its crystal structure at 2.03 A, (Protein Data Bank [PDB] code 2ANE). The structure was solved in two, crystal forms, yielding 14 independent views. The domain exhibits a unique, fold consisting primarily of three twisted beta-sheets and a single long, alpha-helix. Analysis of recent PDB depositions identified a similar fold, in BPP1347 (PDB code 1ZBO), a 203-amino acid protein of unknown function, from Bordetella parapertussis, crystallized as part of a structural, genomics effort. BPP1347 shares sequence homology with Lon N-domains and, with a family of other independently expressed proteins of unknown, functions. We postulate that, as is the case in Lon proteases, this, structural domain represents a general protein and polypeptide interaction, domain.
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<StructureSection load='2ane' size='340' side='right'caption='[[2ane]], [[Resolution|resolution]] 2.03&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2ane]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ANE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ANE FirstGlance]. <br>
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2ANE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/Endopeptidase_La Endopeptidase La], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.53 3.4.21.53] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2ANE OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.03&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ane FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ane OCA], [https://pdbe.org/2ane PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ane RCSB], [https://www.ebi.ac.uk/pdbsum/2ane PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ane ProSAT]</span></td></tr>
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==Reference==
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</table>
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Crystal structure of the N-terminal domain of E. coli Lon protease., Li M, Rasulova F, Melnikov EE, Rotanova TV, Gustchina A, Maurizi MR, Wlodawer A, Protein Sci. 2005 Nov;14(11):2895-900. Epub 2005 Sep 30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16199667 16199667]
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== Function ==
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[[Category: Endopeptidase La]]
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[https://www.uniprot.org/uniprot/LON_ECOLI LON_ECOLI] ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins, including some antitoxins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner. Endogenous substrates include the regulatory proteins RcsA and SulA, the transcriptional activator SoxS, and UmuD. Its overproduction specifically inhibits translation through at least two different pathways, one of them being the YoeB-YefM toxin-antitoxin system.<ref>PMID:12135363</ref> <ref>PMID:16584195</ref> <ref>PMID:19721064</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/an/2ane_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ane ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Gustchina, A.]]
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[[Category: Gustchina A]]
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[[Category: Li, M.]]
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[[Category: Li M]]
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[[Category: Maurizi, M.R.]]
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[[Category: Maurizi MR]]
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[[Category: Melnikov, E.E.]]
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[[Category: Melnikov EE]]
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[[Category: Rasulova, F.]]
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[[Category: Rasulova F]]
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[[Category: Rotanova, T.V.]]
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[[Category: Rotanova TV]]
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[[Category: Wlodawer, A.]]
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[[Category: Wlodawer A]]
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[[Category: lon protease]]
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[[Category: lonn119]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 08:14:29 2007''
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Current revision

Crystal structure of N-terminal domain of E.Coli Lon Protease

PDB ID 2ane

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